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Age-related colonic mucosal microbiome community shifts in monkeys

Citation

Vemuri, R and Sherrill, C and Davis, MA and Kavanagh, K, Age-related colonic mucosal microbiome community shifts in monkeys , Journals of Gerontology. Series A: Biological Sciences and Medical Sciences, 76, (11) pp. 1906-1914. ISSN 1079-5006 (2021) [Refereed Article]

Copyright Statement

Copyright 2020 The Authors

DOI: doi:10.1093/gerona/glaa256

Abstract

Age-related changes in gut microbiome impact host health. The interactive relationship between the microbiome and physiological systems in an aged body system remains to be clearly defined, particularly in the context of inflammation. Therefore, we aimed to evaluate systemic inflammation, microbial translocation (MT), and differences between fecal and mucosal microbiomes. Ascending colon mucosal biopsies, fecal samples, and blood samples from healthy young and old female vervet monkeys were collected for 16S rRNA gene sequencing, MT, and cytokine analyses, respectively. To demonstrate microbial co-occurrence patterns, we used Kendallís tau correlation measure of interactions between microbes. We found elevated levels of plasma LBP-1, MCP-1, and CRP in old monkeys, indicative of higher MT and systemic inflammation. Microbiome analysis revealed significant differences specific to age. At the phylum level, abundances of pathobionts such as Proteobacteria were increased in the mucosa of old monkeys. At the family level, Helicobacteriaceae was highly abundant in mucosal samples (old); in contrast, Ruminococcaceae were higher in the fecal samples of old monkeys. We found significantly lower Firmicutes:Bacteroidetes ratio and lower abundance of butyrate-producing microbes in old monkeys, consistent with less healthy profiles. Microbial community co-occurrence analysis on mucosal samples revealed 13 nodes and 41 associations in the young monkeys, but only 12 nodes and 21 associations in the old monkeys. Our findings provide novel insights into systemic inflammation and gut microbial interactions, highlight the importance of the mucosal niche, and facilitate further understanding of the decline in the stability of the microbial community with aging.

Item Details

Item Type:Refereed Article
Keywords:Aging, microbial co-occurrences, mucosal microbiome, systemic inflammation, cercopithecidae, feces, colon, mucous membrane, community microbiome
Research Division:Biomedical and Clinical Sciences
Research Group:Nutrition and dietetics
Research Field:Nutritional science
Objective Division:Health
Objective Group:Specific population health (excl. Indigenous health)
Objective Field:Health related to ageing
UTAS Author:Kavanagh, K (Associate Professor Kylie Kavanagh)
ID Code:151733
Year Published:2021
Deposited By:Medicine
Deposited On:2022-08-04
Last Modified:2022-09-13
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