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Van de Graaff generator for capillary electrophoresis

Citation

Lee, SJ and Castro, ER and Guijt, RM and Tarn, MD and Manz, A, Van de Graaff generator for capillary electrophoresis, Journal of Chromatography A, 1517 pp. 195-202. ISSN 0021-9673 (2017) [Refereed Article]


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DOI: doi:10.1016/j.chroma.2017.08.026

Abstract

A new approach for high voltage capillary electrophoresis (CE) is proposed, which replaces the standard high voltage power supply with a Van de Graaff generator, a low current power source. Because the Van de Graaff generator is a current-limited source (10 μA), potentials exceeding 100 kV can be generated for CE when the electrical resistance of the capillary is maximized. This was achieved by decreasing the capillary diameter and reducing the buffer ionic strength. Using 2 mM borate buffer and a 5 μm i.d. capillary, fluorescently labeled amino acids were separated with efficiencies up to 3.5 million plates; a 5.7 fold improvement in separation efficiency compared to a normal power supply (NPS) typically used in CE. This separation efficiency was realized using a simple set-up without significant Joule heating, making the Van de Graaff generator a promising alternative for applying the high potentials required for enhancing resolution in the separation and analysis of highly complex samples, for example mixtures of glycans.

Item Details

Item Type:Refereed Article
Keywords:Van de Graaff generator, High Voltage capillary electrophoresis, High separation efficiency, High separation resolution
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Guijt, RM (Dr Rosanne Guijt)
ID Code:151654
Year Published:2017
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2022-08-02
Last Modified:2022-08-02
Downloads:0

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