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Ultraviolet absorbance detector based on a high output power 235 nm surface mounted device-type light-emitting diode
Citation
Lam, SC and Coates, LJ and Gupta, V and Wirth, H-J and Gooley, AA and Haddad, PR and Paull, B, Ultraviolet absorbance detector based on a high output power 235 nm surface mounted device-type light-emitting diode, Journal of Chromatography A, 1631 Article 461540. ISSN 0021-9673 (2020) [Refereed Article]
Copyright Statement
© 2020 Elsevier B.V. All rights reserved.
DOI: doi:10.1016/j.chroma.2020.461540
Abstract
A new miniaturised capillary flow-through deep-UV absorbance detector has been developed using a microscale surface mounted device- type light-emitting diode (LED) (Crystal IS OPTAN 3535-series), emitting at 235 nm and with a half-height band width of 12 nm, and a high-sensitivity Z-shaped flow-cell. Compared with a previously reported TO-39 ball lens LEDs emitting at 235 nm, the new generation LED produced a 20-fold higher optical output and delivered up to 35 times increase in external quantum efficiency (EQE). The Z-cell was based on a reflective rectangular optical path with cross-sectional dimensions of 100 x 100 µm and a physical optical pathlength of 1.2 mm. Inclusion of UV transparent fused-silica ball lenses, between the SMD and the Z-cell, improved light transmission by a factor of 9 and improved the detector signal-to-noise ratio by a factor of 2.2, at the same input current. The detector was housed within an Al-housing fitted with a cooling fan and demonstrated excellent linearity with stray light down to 0.06%, and an effective pathlength of 1.1 mm (92% of nominal pathlength). The resultant detector was fitted successfully into a briefcase-sized portable capillary HPLC system, and practically demonstrated with the detection of a mixture of 13 test compounds at the sub-mg L−1 level in <5 min using gradient elution.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Research Division: | Chemical Sciences |
| Research Group: | Analytical chemistry |
| Research Field: | Separation science |
| Objective Division: | Expanding Knowledge |
| Objective Group: | Expanding knowledge |
| Objective Field: | Expanding knowledge in the chemical sciences |
| UTAS Author: | Lam, SC (Mr Shing Chung Lam) |
| UTAS Author: | Coates, LJ (Mr Lewellwyn Coates) |
| UTAS Author: | Gupta, V (Dr Vipul Gupta) |
| UTAS Author: | Wirth, H-J (Dr Hans-Jurgen Wirth) |
| UTAS Author: | Gooley, AA (Dr Andrew Gooley) |
| UTAS Author: | Haddad, PR (Professor Paul Haddad) |
| UTAS Author: | Paull, B (Professor Brett Paull) |
| ID Code: | 151653 |
| Year Published: | 2020 |
| Web of Science® Times Cited: | 7 |
| Deposited By: | Plant Science |
| Deposited On: | 2022-08-02 |
| Last Modified: | 2022-09-06 |
| Downloads: | 0 |
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