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Miniature multiwavelength deep UV-LED-Based absorption detection system for capillary LC
journal contribution
posted on 2023-05-21, 10:26 authored by Hemida, M, Coates, LJ, Lam, S, Vipul GuptaVipul Gupta, Miroslav MackaMiroslav Macka, Wirth, H-J, Gooley, AA, Paul HaddadPaul HaddadA new miniature deep UV absorbance detector has been developed using low-cost and high-performance LEDs, which can be operated in both scanning (230 to 300 nm) and individual wavelength (240, 255, and 275 nm) detection modes. The detector is mostly composed of off-the-shelf components, such as LEDs, trifurcated fiber optic assembly, a capillary Z-type flow cell, and photodiodes. It has been characterized for use with a standard capillary LC system and was benchmarked against a standard variable wavelength capillary LC detector. The detector shows very low levels of stray light (<0.4%), utilization of up to 99.0% of the effective path length of the flow cell, a wide dynamic range (0.5 to 200 μg/mL for sulfamethazine, carbamazepine, and flavone), and low noise levels (at 300 μAU level). The detector was applied within a miniaturized LC system.
History
Publication title
Analytical ChemistryVolume
92Pagination
13688-13693ISSN
0003-2700Department/School
Tasmanian Institute of Agriculture (TIA)Publisher
Amer Chemical SocPlace of publication
1155 16Th St, Nw, Washington, USA, Dc, 20036Rights statement
Copyright 2020 American Chemical SocietyRepository Status
- Restricted