A new miniature deep UV absorbance detector has been developed using low-cost and high-performance LEDs, which can be operated in both scanning (230 to 300 nm) and individual wavelength (240, 255, and 275 nm) detection modes. The detector is mostly composed of off-the-shelf components, such as LEDs, trifurcated fiber optic assembly, a capillary Z-type flow cell, and photodiodes. It has been characterized for use with a standard capillary LC system and was benchmarked against a standard variable wavelength capillary LC detector. The detector shows very low levels of stray light (<0.4%), utilization of up to 99.0% of the effective path length of the flow cell, a wide dynamic range (0.5 to 200 μg/mL for sulfamethazine, carbamazepine, and flavone), and low noise levels (at 300 μAU level). The detector was applied within a miniaturized LC system.

Item Type:	Refereed Article
Keywords:	Multiwavelength, deep UV absorbance, detection systems, high-performance LEDs, capillary LC
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Instrumental methods (excl. immunological and bioassay methods)
Objective Division:	Economic Framework
Objective Group:	Management and productivity
Objective Field:	Technological and organisational innovation
UTAS Author:	Hemida, M (Mr Mohamed Hemida)
UTAS Author:	Coates, LJ (Mr Lewellwyn Coates)
UTAS Author:	Lam, S (Mr Shing Chung Lam)
UTAS Author:	Gupta, V (Dr Vipul Gupta)
UTAS Author:	Macka, M (Professor Mirek Macka)
UTAS Author:	Haddad, PR (Professor Paul Haddad)
ID Code:	151635
Year Published:	2020
Web of Science® Times Cited:	8
Deposited By:	Chemistry
Deposited On:	2022-08-02
Last Modified:	2022-09-08
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