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Advances in Automated Piston Liquid-Liquid Microextraction Technique


Gras, R and Liu, G and Shellie, RA and Hua, Y and Luong, J, Advances in Automated Piston Liquid-Liquid Microextraction Technique, Journal of Chromatography A, 1651 Article 462330. ISSN 0021-9673 (2021) [Refereed Article]

Copyright Statement

2021 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.chroma.2021.462330


A new automated micro liquid-liquid extraction technique was successfully developed. This novel syringe-based technique capitalizes on the advantages of vigorous fluid agitation and the shearing effect of two fluids with different properties to achieve high extraction efficiency. The technique is at least 20 times faster than mechanical shaking or sonication in achieving a similar recovery even with a hydrophilic probe molecule such as 1,4-dioxane in an aqueous medium. Excellent repeatability with a relative standard deviation as low as 0.56% over a five-day test, n = 2 per day, was demonstrated with 1,4-dioxane. Other model compounds in aqueous matrices evaluated, including phenolics and extraction solvents like chloroform and hexane, showed similar performance in repeatability. An added advantage of this technique involves performing multiple extractions. Its capabilities in conducting complicated extraction steps and minimizing the use of organic solvents as low as 200 L to achieve a preconcentration effect were demonstrated. The technique is suitable for use with emulsion-forming samples without further sample manipulation by incorporating a demulsifier such as acetone during the extraction process. The technique was found to be efficient and environmentally friendly with low solvent waste. This technique is ideal for implementation in automated high throughput and cost-effective quality assurance laboratory environments.

Item Details

Item Type:Refereed Article
Keywords:Liquid-liquid extraction, Mixxor, Emulsion-forming, 1,4-dioxane, demulsifier, gas chromatography
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Gras, R (Ms Ronda Gras)
UTAS Author:Shellie, RA (Professor Robert Shellie)
UTAS Author:Luong, J (Mr Jim Luong)
ID Code:151564
Year Published:2021
Web of Science® Times Cited:1
Deposited By:Plant Science
Deposited On:2022-08-01
Last Modified:2022-10-27

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