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Modular, cost-effective, and portable capillary gradient liquid chromatography system for on-site analysis


Coates, LJ and Lam, SC and Gooley, AA and Haddad, PR and Paull, B and Wirth, H-J, Modular, cost-effective, and portable capillary gradient liquid chromatography system for on-site analysis, Journal of Chromatography A, 1626 Article 461374. ISSN 0021-9673 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 Elsevier B.V

DOI: doi:10.1016/j.chroma.2020.461374


This work demonstrates the development of a compact, modular, cost-effective separation system configured to address a specific separation problem. The principles of the separation are based on gradient capillary liquid chromatography where the system consists of precision stepper motor-driven portable syringe pumps with interchangeable glass syringes (100 無 to 1000 無). Excellent flow-rate precision of ≤ 1% RSD was achieved with typical flow-rates ranging from 1 無/min to 100 無/min, which was ideal for capillary columns. A variable external loop volume and electrically actuated miniature injection valve was used for sample introduction. Detection was based upon a commercial Z-type UV absorbance flow-cell housed within a custom-built cooling enclosure (40 mm x 40 mm) which also contained a UV-LED light-source and a photodiode. System and chromatographic performance was evaluated using linear gradient elution, with day to day repeatability of <1.5% RSD (n = 6) for peak area, and < 0.4% RSD (n = 6) for retention time, for the separation of a 5 component mixture using a 50 mm X 530 痠 ID C18 3 痠 particle capillary column. The system can run any commercial or in-house packed columns from 50 mm to 100 mm length with IDs ranging from 200 to 700 痠. The developed portable system was operated using custom-built windows-based chromatography software, complete with data acquisition and system control.

Item Details

Item Type:Refereed Article
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Coates, LJ (Mr Lewellwyn Coates)
UTAS Author:Lam, SC (Mr Shing Chung Lam)
UTAS Author:Gooley, AA (Dr Andrew Gooley)
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Paull, B (Professor Brett Paull)
UTAS Author:Wirth, H-J (Dr Hans-Jurgen Wirth)
ID Code:151562
Year Published:2020
Web of Science® Times Cited:14
Deposited By:Plant Science
Deposited On:2022-08-01
Last Modified:2022-09-12

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