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Prediction of analyte retention time in liquid chromatography


Haddad, PR and Taraji, M and Szucs, R, Prediction of analyte retention time in liquid chromatography, Analytical Chemistry, 93 pp. 228-256. ISSN 0003-2700 (2021) [Substantial Review]

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DOI: doi:10.1021/acs.analchem.0c04190


This review addresses the approaches used to predict chromatographic retention time in a range of liquid chromatographic techniques, with a particular focus on reversed-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), and ion chromatography (IC). This focus has been selected on the basis that prediction of retention for these three chromatographic techniques comprises the bulk of the literature and also because the approaches used differ markedly between these techniques. The literature for this review is drawn predominantly from the period 20152020. Many different types of analytes are included in the review, with the exception of peptides and proteins, which are omitted for two main reasons. First, the methodology used for the prediction of retention of peptides and proteins differs significantly from that used for small molecules in that retention is often assumed to derive from the sequence of constituent amino acids, and this approach is not relevant to small molecules. Second, the prediction of retention for proteins and peptides has been the subject of a number of reviews

Item Details

Item Type:Substantial Review
Keywords:Chromatography, metabolism, pharmaceuticals, separation science, solvents
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Taraji, M (Ms Maryam Taraji)
UTAS Author:Szucs, R (Dr Roman Szucs)
ID Code:151556
Year Published:2021
Deposited By:Chemistry
Deposited On:2022-08-01
Last Modified:2022-08-01

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