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Miniature and fully portable gradient capillary liquid chromatograph


Lam, SC and Coates, LJ and Hemida, MH and Gupta, V and Haddad, PR and Macka, M and Paull, B, Miniature and fully portable gradient capillary liquid chromatograph, Analytica Chimica Acta: International Journal Devoted to All Branches of Analytical Chemistry, 1101 pp. 199-210. ISSN 0003-2670 (2020) [Refereed Article]

Copyright Statement

© 2019 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.aca.2019.12.014


A robust, portable and miniature battery powered gradient capillary liquid chromatograph (total weight ~2.7 kg, without battery ~2.0 kg), with integrated microfluidic injection, column heating and high sensitivity low-UV absorbance detection is presented. The portable capillary chromatograph, was applied with a packed reversed-phase capillary column (100 mm x 300 μm I.D., 5 μm ODS), housed within an integrated capillary column heater controlled by a proportional-integral-derivative (PID) chip module. The system delivered retention time and peak area relative standard deviation in isocratic mode of <0.7% (n = 10) and <3.3% (n = 10), respectively, and <0.1% (n = 10) and <2.3% (n = 10) respectively, for gradient elution mode. Detection was based upon a 255 nm light-emitting diode (LED) using one of two commercial capillary flow-cell options, namely a high sensitivity 12 nL Agilent capillary z-cell (HSDC) and a 45 nL Thermo Fisher Scientific UZ-View™ flow cell (UZFC). The HSDC, housed within a 3D printed detector arrangement, gave an effective pathlength of 1.01 mm (84% of nominal pathlength) and stray light of only 0.2%. Limits of detection for four test small molecule pharmaceuticals ranged from 65 to 101 μg L−1 based upon a 316 nL injection volume, with separation efficiencies of between 18,000 and 29,700 N m−1, with sub-4 min run times. The portable capillary LC system was successfully coupled to a small footprint portable mass spectrometer (Microsaic 4500 MiD) to demonstrate compatibility and ‘point-of-need’ miniaturised LC-MS capability.

Item Details

Item Type:Refereed Article
Keywords:Miniaturisation, portable, capillary liquid chromatography, ultraviolet light-emitting diode-based detector, column heater, portable mass spectrometer
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Lam, SC (Mr Shing Chung Lam)
UTAS Author:Coates, LJ (Mr Lewellwyn Coates)
UTAS Author:Hemida, MH (Mr Mohamed Hemida)
UTAS Author:Gupta, V (Dr Vipul Gupta)
UTAS Author:Haddad, PR (Professor Paul Haddad)
UTAS Author:Macka, M (Professor Mirek Macka)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:151504
Year Published:2020
Funding Support:Australian Research Council (IC140100022)
Web of Science® Times Cited:26
Deposited By:Chemistry
Deposited On:2022-08-01
Last Modified:2022-09-01

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