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The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research


du Sert, NP and Hurst, V and Ahluwalia, A and Alam, S and Avey, MT and Baker, M and Browne, WJ and Clark, A and Cuthill, IC and Dirnagl, U and Emerson, M and Garner, P and Holgate, ST and Howells, DW and Karp, NA and Lazic, SE and Lidster, K and MacCallum, CJ and Macleod, M and Pearl, EJ and Petersen, OH and Rawle, F and Reynolds, P and Rooney, K and Sena, ES and Silberberg, SD and Steckler, T and Wurbel, H, The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research, PLoS Biology, 18, (7) Article e3000410. ISSN 1544-9173 (2020) [Refereed Article]

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Copyright Statement

Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 1.0 Universal (CC0 1.0) public domain dedication. (

DOI: doi:10.1371/journal.pbio.3000411


Reproducible science requires transparent reporting. The ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments) were originally developed in 2010 to improve the reporting of animal research. They consist of a checklist of information to include in publications describing in vivo experiments to enable others to scrutinise the work adequately, evaluate its methodological rigour, and reproduce the methods and results. Despite considerable levels of endorsement by funders and journals over the years, adherence to the guidelines has been inconsistent, and the anticipated improvements in the quality of reporting in animal research publications have not been achieved. Here, we introduce ARRIVE 2.0. The guidelines have been updated and information reorganised to facilitate their use in practice. We used a Delphi exercise to prioritise and divide the items of the guidelines into 2 sets, the "ARRIVE Essential 10," which constitutes the minimum requirement, and the "Recommended Set," which describes the research context. This division facilitates improved reporting of animal research by supporting a stepwise approach to implementation. This helps journal editors and reviewers verify that the most important items are being reported in manuscripts. We have also developed the accompanying Explanation and Elaboration (E&E) document, which serves (1) to explain the rationale behind each item in the guidelines, (2) to clarify key concepts, and (3) to provide illustrative examples. We aim, through these changes, to help ensure that researchers, reviewers, and journal editors are better equipped to improve the rigour and transparency of the scientific process and thus reproducibility.

Item Details

Item Type:Refereed Article
Keywords:ARRIVE guidelines, reproducible science, animal research, transparent reporting
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Clinical sciences not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Howells, DW (Professor David Howells)
ID Code:151466
Year Published:2020
Web of Science® Times Cited:646
Deposited By:Medicine
Deposited On:2022-07-29
Last Modified:2022-08-16
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