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Inhibition of T-type calcium channels suppresses cell proliferation and invasion and promotes apoptosis in human medulloblastoma cell lines [Poster]

Citation

Sedeeq, M and Maklad, A and Azimi, I, Inhibition of T-type calcium channels suppresses cell proliferation and invasion and promotes apoptosis in human medulloblastoma cell lines [Poster], 33rd Lorne Cancer Conference, 11-13 February 2021, Online Web Conference (2021) [Conference Extract]


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Official URL: http://cancer-2021.p.asnevents.com.au/days/2021-02...

Abstract

Medulloblastoma (MB) is the most common malignant childhood brain cancer. The low efficacy and long-lasting side effects of current therapies necessitate the need for a new treatment for MB. In this study, in-silico transcriptomics analysis of MB patients revealed a significant correlation of a CaV3 calcium channel expression with metastasis and patient survival rates. Pharmacological inhibition of T-type calcium channels by mibefradil or NNC-55-0396 (NNC) significantly reduced MB cell proliferation and resistance to MB chemotherapeutic agent, vincristine, as assessed by WST-1 proliferation assay. Mibefradil or NNC treatment further reduced invasive abilities of MB cells in an agar invasion assay. These responses were specific to T-type calcium channel blockade as inhibition of L-type channels did not produce the same effects. Further studies revealed an induction of apoptosis by mibefradil or NNC treatment in a concentration-dependent manner. Our results present the first evidence to the antitumor effects of mibefradil and NNC in medulloblastoma cells. This study provides insight into the future development of potential therapeutic approaches for MB that target the invasion, proliferation, and drug resistance in these cells.

Item Details

Item Type:Conference Extract
Keywords:Medulloblastoma, T-type calcium channels, Mibefradil, NNC-550396, Metabolic activity, Apoptosis
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Basic pharmacology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Sedeeq, M (Mr Mohammed Sedeeq)
UTAS Author:Maklad, A (Mr Ahmed Maklad)
UTAS Author:Azimi, I (Dr Iman Azimi)
ID Code:151394
Year Published:2021
Deposited By:Pharmacy
Deposited On:2022-07-28
Last Modified:2022-07-28
Downloads:0

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