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Role of reactive oxygen species in calcium signalling in hypoxia-induced epithelial-mesenchymal transition [Poster]


Azimi, I and Thompson, EW and Roberts-Thomson, SJ and Monteith, GR, Role of reactive oxygen species in calcium signalling in hypoxia-induced epithelial-mesenchymal transition [Poster], Australasian Society of Clinical and Experimental Pharmacologists and Toxicologists Annual Scientific Meeting (ASCEPT2013), 1-4 December 2013, Melbourne, Australia, pp. 117. (2013) [Conference Extract]

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Introduction: Hypoxia is a hallmark of the cancer microenvironment and induces epithelial-mesenchymal transition (EMT) in breast cancer cells. EMT describes the transition of breast cancer cells into a more invasive phenotype. Hypoxia has been reported to cause changes in calcium (Ca2+) signalling in a variety of cell types including osteosarcoma and HEK293 cells, possibly through the production of reactive oxygen species (ROS), however, this pathway has not been studied in breast cancer cells.

Aims: To investigate the role of hypoxia-mediated increases in ROS in the induction of EMT and altered Ca2+ signalling in MDA-MB-468 breast cancer cells.

Methods: MDA-MB-468 breast cancer cells were incubated for 24 h at 1% O2 to induce hypoxia. ROS increases were assessed using a cell-permeable fluorogenic probe 2,7-dichlorodihydrofluorescin diacetate (DCFH-DA). Real time RT-PCR was used to assess the mRNA levels of 50 calcium channels and transporters in MDA-MB-468 cells in the presence and absence of hypoxia.

Results: Hypoxia increased levels of the EMT markers vimentin and N-cadherin, and increased intracellular ROS levels. Five Ca2+ permeable channels belonging to different classes were identified as altered by hypoxia in MDAMB- 468 breast cancer cells. Chelation of ROS with 10 mM N-acetylcysteine (NAC) significantly changed the expression pattern of these five selected Ca2+ channels as well as the EMT marker N-cadherin.

Discussion: These results suggest an important role of ROS in hypoxia-mediated EMT in breast cancer cells and indicate that such changes are associated with alterations in the expression of specific Ca2+ permeable ion channels.

Item Details

Item Type:Conference Extract
Keywords:reactive oxygen species, calcium signalling, hypoxia, epithelial-mesenchymal transition
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Basic pharmacology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Azimi, I (Dr Iman Azimi)
ID Code:151208
Year Published:2013
Deposited By:Pharmacy
Deposited On:2022-07-25
Last Modified:2022-07-28

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