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ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid

Citation

Liu, X and Hu, Q and Yan, J and Sun, K and Liang, Y and Jia, M and Meng, X and Fang, S and Wang, Y and Jing, Y and Liu, G and Wu, D and Chu, C and Smith, SM and Chu, J and Wang, Y and Li, J and Wang, B, ζ-Carotene Isomerase Suppresses Tillering in Rice through the Coordinated Biosynthesis of Strigolactone and Abscisic Acid, Molecular Plant, 13, (12) pp. 1784-1801. ISSN 1674-2052 (2020) [Refereed Article]

DOI: doi:10.1016/j.molp.2020.10.001

Abstract

Rice tillering is an important agronomic trait affecting grain yield. Here, we identified a high-tillering mutant tillering20 (t20), which could be restored to the wild type by treatment with the strigolactone (SL) analog rac-GR24. T20 encodes a chloroplast ζ-carotene isomerase (Z-ISO), which is involved in the biosynthesis of carotenoids and their metabolites, SL and abscisic acid (ABA). The t20 mutant has reduced SL and ABA, raising the question of how SL and ABA biosynthesis is coordinated, and whether they have overlapping functions in tillering. We discovered that rac-GR24 stimulated T20 expression and enhanced all-trans-β-carotene biosynthesis. Importantly, rac-GR24 also stimulated expression of Oryza sativa 9-CIS-EPOXYCAROTENOID DIOXYGENASE 1 (OsNCED1) through induction of Oryza sativa HOMEOBOX12 (OsHOX12), promoting ABA biosynthesis in shoot base. On the other hand, ABA treatment significantly repressed SL biosynthesis and the ABA biosynthetic mutants displayed elevated SL biosynthesis. ABA treatment reduced the number of basal tillers in both t20 and wild-type plants. Furthermore, while ABA-deficient mutants aba1 and aba2 had the same number of basal tillers as wild type, they had more unproductive upper tillers at maturity. This work demonstrates complex interactions in the biosynthesis of carotenoid, SLs and ABA, and reveals a role for ABA in the regulation of rice tillering.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Rice
UTAS Author:Smith, SM (Professor Steven Smith)
ID Code:151164
Year Published:2020
Web of Science® Times Cited:23
Deposited By:Plant Science
Deposited On:2022-07-23
Last Modified:2022-07-23
Downloads:0

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