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Primordial germ cell development in the poeciliid, Gambusia holbrooki, reveals shared features between lecithotrophs and matrotrophs

Citation

Razmi, K and Patil, JG, Primordial germ cell development in the poeciliid, Gambusia holbrooki, reveals shared features between lecithotrophs and matrotrophs, Frontiers in Cell and Developmental Biology, 10 Article 793498. ISSN 2296-634X (2022) [Refereed Article]


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Copyright Statement

Copyright 2022 Razmi and Patil Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3389/fcell.2022.793498

Abstract

Metazoans exhibit two modes of primordial germ cell (PGC) specification that are interspersed across taxa. However, the evolutionary link between the two modes and the reproductive strategies of lecithotrophy and matrotrophy is poorly understood. As a first step to understand this, the spatio-temporal expression of teleostean germ plasm markers was investigated in Gambusia holbrooki, a poecilid with shared lecitho- and matrotrophy. A group of germ plasm components was detected in the ovum suggesting maternal inheritance mode of PGC specification. However, the strictly zygotic activation of dnd-β and nanos1 occurred relatively early, reminiscent of models with induction mode (e.g., mice). The PGC clustering, migration and colonisation patterns of G. holbrooki resembled those of zebrafish, medaka and mice at blastula, gastrula and somitogenesis, respectively—recapitulating features of advancing evolutionary nodes with progressive developmental stages. Moreover, the expression domains of PGC markers in G. holbrooki were either specific to teleost (vasa expression in developing PGCs), murine models (dnd spliced variants) or shared between the two taxa (germline and somatic expression of piwi and nanos1). Collectively, the results suggest that the reproductive developmental adaptations may reflect a transition from lecithotrophy to matrotrophy.

Item Details

Item Type:Refereed Article
Keywords:Gambusia, germ cell, development
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish physiology and genetics
Objective Division:Environmental Management
Objective Group:Fresh, ground and surface water systems and management
Objective Field:Control of pests, diseases and exotic species in fresh, ground and surface water
UTAS Author:Razmi, K (Mr Komeil Razmi)
UTAS Author:Patil, JG (Dr Jawahar PATIL)
ID Code:149848
Year Published:2022
Funding Support:Australian Research Council (LP140100428)
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2022-04-18
Last Modified:2022-05-06
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