149745 - Capsaicin and zinc promote glucose uptake in C2C12 skeletal muscle cells through a common calcium signalling pathway.pdf (2.52 MB)
Capsaicin and zinc promote glucose uptake in C2C12 skeletal muscle cells through a common calcium signalling pathway
journal contribution
posted on 2023-05-21, 07:09 authored by Vahidi Ferdowsi, P, Kiran AhujaKiran Ahuja, Jeffrey BeckettJeffrey Beckett, Stephen MyersStephen MyersCapsaicin and zinc have recently been highlighted as potential treatments for glucose metabolism disorders; however, the effect of these two natural compounds on signalling pathways involved in glucose metabolism is still uncertain. In this study, we assessed the capsaicin- or zinc- induced activation of signalling molecules including calcium/calmodulin-dependent protein kinase 2 (CAMKK2), cAMP-response element-binding protein (CREB), and target of rapamycin kinase complex 1 (TORC1). Moreover, the expression status of genes associated with the control of glucose metabolism was measured in treated cells. The activation of cell signalling proteins was then evaluated in capsaicin- or zinc treated cells in the presence or absence of cell-permeant calcium chelator (BAPTA-AM) and the CAMKK inhibitor (STO-609). Finally, capsaicin- and zinc-induced glucose uptake was measured in the cells pre-treated with or without BAPTA-AM. Our results indicate that calcium flux induced by capsaicin or zinc led to activation of calcium signalling molecules and promoting glucose uptake in skeletal muscle cells. Pharmacological inhibition of CAMKK diminished activation of signalling molecules. Moreover, we observed an increase in intracellular cAMP levels in the cells after treatment with capsaicin and zinc. Our data show that capsaicin and zinc mediate glucose uptake in C2C12 skeletal muscle cells through the activation of calcium signalling.
History
Publication title
International Journal of Molecular SciencesVolume
23Issue
4Pagination
2207ISSN
1422-0067Department/School
School of Health SciencesPublisher
MDPI AGPlace of publication
SwitzerlandRights statement
Copyright 2022 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/Repository Status
- Open