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Capsaicin and zinc promote glucose uptake in C2C12 skeletal muscle cells through a common calcium signalling pathway

Citation

Vahidi Ferdowsi, P and Ahuja, KDK and Beckett, JM and Myers, S, Capsaicin and zinc promote glucose uptake in C2C12 skeletal muscle cells through a common calcium signalling pathway, International Journal of Molecular Sciences, 23, (4) pp. 2207. ISSN 1422-0067 (2022) [Refereed Article]


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Copyright 2022 The Authors Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms23042207

Abstract

Capsaicin and zinc have recently been highlighted as potential treatments for glucose metabolism disorders; however, the effect of these two natural compounds on signalling pathways involved in glucose metabolism is still uncertain. In this study, we assessed the capsaicin- or zinc- induced activation of signalling molecules including calcium/calmodulin-dependent protein kinase 2 (CAMKK2), cAMP-response element-binding protein (CREB), and target of rapamycin kinase complex 1 (TORC1). Moreover, the expression status of genes associated with the control of glucose metabolism was measured in treated cells. The activation of cell signalling proteins was then evaluated in capsaicin- or zinc treated cells in the presence or absence of cell-permeant calcium chelator (BAPTA-AM) and the CAMKK inhibitor (STO-609). Finally, capsaicin- and zinc-induced glucose uptake was measured in the cells pre-treated with or without BAPTA-AM. Our results indicate that calcium flux induced by capsaicin or zinc led to activation of calcium signalling molecules and promoting glucose uptake in skeletal muscle cells. Pharmacological inhibition of CAMKK diminished activation of signalling molecules. Moreover, we observed an increase in intracellular cAMP levels in the cells after treatment with capsaicin and zinc. Our data show that capsaicin and zinc mediate glucose uptake in C2C12 skeletal muscle cells through the activation of calcium signalling.

Item Details

Item Type:Refereed Article
Keywords:glucose metabolism, CAMKK2, CREB, TORC1, Nr4a3, Junb, calcium flux, cAMP signalling
Research Division:Biomedical and Clinical Sciences
Research Group:Clinical sciences
Research Field:Clinical sciences not elsewhere classified
Objective Division:Health
Objective Group:Other health
Objective Field:Other health not elsewhere classified
UTAS Author:Vahidi Ferdowsi, P (Ms Parisa Vahidi Ferdowsi)
UTAS Author:Ahuja, KDK (Dr Kiran Ahuja)
UTAS Author:Beckett, JM (Dr Jeff Beckett)
UTAS Author:Myers, S (Dr Stephen Myers)
ID Code:149745
Year Published:2022
Deposited By:Health Sciences
Deposited On:2022-04-09
Last Modified:2022-05-06
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