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IgG fractions from patients with antiphospholipid syndrome and systemic lupus erythematosus bind to platelets, but do not affect collagen-induced platelet activation
Citation
Ho, YC and Brake, SJ and Ahuja, KDK and Acott, N and Tiao, J and Baker, R and Adams, MJ, IgG fractions from patients with antiphospholipid syndrome and systemic lupus erythematosus bind to platelets, but do not affect collagen-induced platelet activation, Biotechnic and Histochemistry, 97, (8) pp. 604-615. ISSN 1052-0295 (2022) [Refereed Article]
Copyright Statement
Copyright 2022 The Biological Stain Commission.
DOI: doi:10.1080/10520295.2022.2049878
Abstract
Anti-beta-2 glycoprotein 1 (anti-β2GP1) is an antiphospholipid antibody found in patients with antiphospholipid syndrome (APS) and systemic lupus erythematosus (SLE). Its presence commonly is associated with thrombosis; however, the mechanisms of interaction of anti-β2GP1 antibodies and platelets remain unclear. We investigated the effects of APS and SLE patient-derived IgG fractions on collagen-mediated platelet aggregation and examined the binding of patient-derived IgG to platelets before and after activation by collagen. IgG fractions, 150, 200, 300 or 350 µg/ml, isolated from 11 patients with APS and SLE were incubated with two sets of platelet-rich plasma (PRP) in the incubation wells of an aggregometer. The first set was activated by collagen and the other set was incubated for an additional 10 min. All platelets were collected by centrifugation and fixed in cell blocks. We assessed binding of IgG to platelets using immunocytochemistry (ICC). Patient-derived IgG fractions did not affect collagen-induced platelet aggregation. ICC staining using anti-human IgG antibodies demonstrated that patient-derived IgG fractions had greater affinity for non-activated platelets than those activated by 0.75 µg/ml collagen. Patient-derived IgG fractions bound to the surface of platelets and potentially could be internalized by platelets. IgG fractions from APS and SLE patients may sensitize non-activated platelets, which could increase platelet reactivity and thrombotic risk in patients. We did not detect secondary effects of patient-derived IgG fractions.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | lupus |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Cardiovascular medicine and haematology |
| Research Field: | Haematology |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Treatment of human diseases and conditions |
| UTAS Author: | Ho, YC (Mr Yik Ho) |
| UTAS Author: | Brake, SJ (Mr Sam Brake) |
| UTAS Author: | Ahuja, KDK (Dr Kiran Ahuja) |
| ID Code: | 149744 |
| Year Published: | 2022 |
| Deposited By: | Health Sciences |
| Deposited On: | 2022-04-09 |
| Last Modified: | 2022-12-23 |
| Downloads: | 0 |
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