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Genetic analysis of early phenology in lentil identifies distinct loci controlling component traits

Citation

Rajandran, V and Ortega, R and Vander Schoor, JK and Butler, JB and Freeman, JS and Hecht, VFG and Erskine, W and Murfet, IC and Bett, KE and Weller, JL, Genetic analysis of early phenology in lentil identifies distinct loci controlling component traits, Journal of Experimental Botany, 73, (12) pp. 3963-3977. ISSN 0022-0957 (2022) [Refereed Article]


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Copyright Statement

© The Author(s) 2022. This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Experimental Botany following peer review. The version of record, Vinodan Rajandran, Raul Ortega, Jacqueline K Vander Schoor, Jakob B Butler, Jules S Freeman, Valerie F G Hecht, Willie Erskine, Ian C Murfet, Kirstin E Bett, James L Weller, Genetic analysis of early phenology in lentil identifies distinct loci controlling component traits, Journal of Experimental Botany, 2022, erac107, is available online at: https://doi.org/10.1093/jxb/erac107

DOI: doi:10.1093/jxb/erac107

Abstract

Modern-day domesticated lentil germplasm is generally considered to form three broad adaptation groups: Mediterranean, South Asian and northern temperate, which correspond to the major global production environments. Reproductive phenology plays a key role in lentil adaptation to this diverse ecogeographic variation. Here, we dissect the characteristic earliness of the pilosae ecotype, suited to the typically short cropping season of South Asian environments. We identified two loci, DTF6a and DTF6b, at which dominant alleles confer early flowering, and we show that DTF6a alone is sufficient to confer early flowering under extremely short photoperiods. Genomic synteny confirmed the presence of a conserved cluster of three florigen (FT) gene orthologs among potential candidate genes, and expression analysis in near-isogenic material showed that the early allele is associated with a strong derepression of the FTa1 gene in particular. Sequence analysis revealed a 7.4 kb deletion in the FTa1-FTa2 intergenic region in the pilosae parent, and a wide survey of over 350 accessions with diverse origin showed that the dtf6a allele is predominant in South Asian material. Collectively, these results contribute to understanding the molecular basis of global adaptation in lentil, and further emphasize the importance of this conserved genomic region for adaptation in temperate legumes generally.

Item Details

Item Type:Refereed Article
Keywords:adaptation, controlled environment, flowering time, FT genes, photoperiod, legume, lentil, mapping, QTL analysis, florigen, lentil, phenology
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant developmental and reproductive biology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biological sciences
UTAS Author:Rajandran, V (Mr Vinodan Rajandran)
UTAS Author:Ortega, R (Mr Raul Ortega Martinez)
UTAS Author:Vander Schoor, JK (Mrs Jacqueline Vander Schoor)
UTAS Author:Butler, JB (Dr Jakob Butler)
UTAS Author:Freeman, JS (Dr Jules Freeman)
UTAS Author:Hecht, VFG (Dr Valerie Hecht)
UTAS Author:Murfet, IC (Professor Ian Murfet)
UTAS Author:Weller, JL (Associate Professor Jim Weller)
ID Code:149701
Year Published:2022
Web of Science® Times Cited:3
Deposited By:Plant Science
Deposited On:2022-04-07
Last Modified:2022-12-09
Downloads:0

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