eCite Digital Repository

The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock


Bommana, S and Jelocnik, M and Borel, N and Marsh, I and Carver, S and Polkinghorne, A, The limitations of commercial serological assays for detection of chlamydial infections in Australian livestock, Journal of Medical Microbiology, 68, (4) pp. 627-632. ISSN 0022-2615 (2019) [Refereed Article]

Restricted - Request a copy

Copyright Statement

Copyright 2019 The Authors

DOI: doi:10.1099/jmm.0.000951


Chlamydia pecorumand Chlamydia abortus are related ruminant pathogens endemic to different global regions. Potential co-infections combined with the lack of species-specific serological assays challenge accurate diagnosis. Serological screening revealed low C. abortus seropositivity with the peptide-based ELISA (1/84; 1.2%) in Australian sheep yet moderate seropositivity in a Swiss flock with history of C. abortus-associated abortions (17/63; 26.9%). By whole cell antigen complement fixation tests (CFT) and ELISA, chlamydial seropositivity was significantly higher in all groups, suggesting cross-reactivity between these two chlamydial species and non-specificity of the tests. However, only C. pecorum DNA could be detected by qPCR in Chlamydia seropositive Australian animals screened, suggesting chlamydial seropositivity was due to cross-reactivity with endemic C. pecorum infections. These results suggest ascribing Chlamydia seropositivity to chlamydial species in livestock using whole-cell antigen CFT or ELISA should be treated with caution; and that peptide-based ELISA and qPCR provide greater chlamydial species-specificity.

Item Details

Item Type:Refereed Article
Keywords:Chlamydia abortus , Chlamydia pecorum , ovine enzootic abortion, serology, whole antigen based ELISA, peptide antigen based ELISA, epidemiology
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Veterinary sciences
Research Field:Veterinary parasitology
Objective Division:Environmental Management
Objective Group:Terrestrial systems and management
Objective Field:Control of pests, diseases and exotic species in terrestrial environments
UTAS Author:Carver, S (Associate Professor Scott Carver)
ID Code:149645
Year Published:2019
Funding Support:Australian Research Council (LP140100315)
Web of Science® Times Cited:8
Deposited By:Zoology
Deposited On:2022-04-05
Last Modified:2022-05-05

Repository Staff Only: item control page