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Characterization and genomic analysis of the first podophage infecting Shewanella, representing a novel viral cluster

Citation

Dong, Y and Zheng, K and Zou, X and Liang, Y and Liu, Y and Li, X and Shao, H and Sung, YY and Mok, WJ and Wong, LL and McMinn, A and Wang, M, Characterization and genomic analysis of the first podophage infecting Shewanella, representing a novel viral cluster, Frontiers in Microbiology, 13 Article 853973. ISSN 1664-302X (2022) [Refereed Article]


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Copyright Statement

Copyright © 2022 Dong, Zheng, Zou, Liang, Liu, Li, Shao, Sung, Mok, Wong, McMinn and Wang. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

DOI: doi:10.3389/fmicb.2022.853973

Abstract

Shewanella is a common bacterial genus in marine sediments and deep seas, with a variety of metabolic abilities, suggesting its important roles in the marine biogeochemical cycles. In this study, a novel lytic Shewanella phage, vB_SInP-X14, was isolated from the surface coastal waters of Qingdao, China. The vB_SInP-X14 contains a linear, double-strand 36,396-bp with the G + C content of 44.1% and harbors 40 predicted open reading frames. Morphological, growth, and genomic analysis showed that it is the first isolated podovirus infecting Shewanella, with a short propagation time (40 min), which might be resulted from three lytic-related genes. Phylogenetic analysis suggested that vB_SInP-X14 could represent a novel viral genus, named Bocovirus, with four isolated but not classified phages. In addition, 14 uncultured viral genomes assembled from the marine metagenomes could provide additional support to establish this novel viral genus. This study reports the first podovirus infecting Shewanella, establishes a new interaction system for the study of virus–host interactions, and also provides new reference genomes for the marine viral metagenomic analysis.

Item Details

Item Type:Refereed Article
Keywords:Shewanella, podophage, genomic analysis, phylogenetic analysis, novel viral cluster
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Virology
Objective Division:Environmental Management
Objective Group:Coastal and estuarine systems and management
Objective Field:Coastal or estuarine biodiversity
UTAS Author:McMinn, A (Professor Andrew McMinn)
ID Code:149509
Year Published:2022
Deposited By:Ecology and Biodiversity
Deposited On:2022-04-03
Last Modified:2022-05-13
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