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Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle

Citation

Lo, HP and Lim, Y-W and Xiong, Z and Martel, N and Ferguson, C and Ariotti, N and Giacomotto, J and Rae, J and Floetenmeyer, M and Moradi, SV and Gao, Y and Tillu, VA and Xia, D and Wang, H and Rahnama, S and Nixon, SJ and Bastiani, M and Day, RD and Smith, KA and Palpant, NJ and Johnston, WA and Alexandrov, K and Collins, BM and Hall, TE and Parton, RG, Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle, Journal of Cell Biology, 220, (12) Article e201905065. ISSN 0021-9525 (2021) [Refereed Article]


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© 2021 Lo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

DOI: doi:10.1083/jcb.201905065

Abstract

The cavin proteins are essential for caveola biogenesis and function. Here, we identify a role for the muscle-specific component, Cavin4, in skeletal muscle T-tubule development by analyzing two vertebrate systems, mouse and zebrafish. In both models, Cavin4 localized to T-tubules, and loss of Cavin4 resulted in aberrant T-tubule maturation. In zebrafish, which possess duplicated cavin4 paralogs, Cavin4b was shown to directly interact with the T-tubule-associated BAR domain protein Bin1. Loss of both Cavin4a and Cavin4b caused aberrant accumulation of interconnected caveolae within the T-tubules, a fragmented T-tubule network enriched in Caveolin-3, and an impaired Ca2+ response upon mechanical stimulation. We propose a role for Cavin4 in remodeling the T-tubule membrane early in development by recycling caveolar components from the T-tubule to the sarcolemma. This generates a stable T-tubule domain lacking caveolae that is essential for T-tubule function.

Item Details

Item Type:Refereed Article
Keywords:skeletal muscle, cavin proteins, Cavin4
Research Division:Biological Sciences
Research Group:Zoology
Research Field:Animal physiology - cell
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the biomedical and clinical sciences
UTAS Author:Day, RD (Dr Ryan Day)
ID Code:149496
Year Published:2021
Web of Science® Times Cited:1
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2022-04-01
Last Modified:2022-05-19
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