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Genetic identification of asteroid larvae from Tasmania, Australia, by PCR-RFLP


Evans, BS and White, RWG and Ward, RD, Genetic identification of asteroid larvae from Tasmania, Australia, by PCR-RFLP, Molecular Ecology, 7, (8) pp. 1077-1082. ISSN 0962-1083 (1998) [Refereed Article]

DOI: doi:10.1046/j.1365-294x.1998.00395.x


Studies of seastar larvae in the Derwent River (Tasmania) were hampered by identification uncertainties. A genetic test was developed, based on PCR amplification of a 1300 bp mitochondrial DNA region followed by digestion with three restriction enzymes. The restriction profiles of adults of 14 seastar species were determined. The test was validated for larvae as laboratory-raised larvae of two species had the appropriate composite haplotypes. Approximately 80% of planktotrophic seastar larvae from Derwent River plankton samples were identified as the recently introduced northern Pacific seastar, Asterias amurensis. The two other larval seastars identified were Coscinasterias muricata and Patiriella regularis.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Evolutionary biology
Research Field:Animal systematics and taxonomy
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the environmental sciences
UTAS Author:Evans, BS (Mr Bradley Scott Evans)
UTAS Author:White, RWG (Professor Rob White)
ID Code:14944
Year Published:1998
Web of Science® Times Cited:30
Deposited By:Zoology
Deposited On:1998-08-01
Last Modified:2011-08-09

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