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Genetic mapping in pea. 2. Identification of RAPD and SCAR markers linked to genes affecting plant architecture

Citation

Rameau, C and Denoue, D and Fraval, F and Haurogne, K and Josserand, J and Laucou, V and Batge, SL and Murfet, IC, Genetic mapping in pea. 2. Identification of RAPD and SCAR markers linked to genes affecting plant architecture, Theoretical and Applied Genetetics, 97, (5-6) pp. 916-928. ISSN 0040-5752 (1998) [Refereed Article]

DOI: doi:10.1007/s001220050972

Abstract

Random amplified polymorphic DNA (RAPD) markers linked to two morphological markers (fa and det), three ramosus genes (rms2, rms3 and rms4) and two genes conferring flowering response to photoperiod in pea (sn, dne) were selected by bulk segregant analysis on F2 populations. Two RAPD fragments were cloned and sequenced to generate the two SCAR markers V20 and S2 which are linked to rms3 and dne, respectively. All these genes, except rms2, were previously located on the pea classical linkage map. Rms2 mapped to linkage group IB which contains the afila gene. Precise genetic maps of the regions containing the genes were obtained and compared to the RAPD map generated from the recombinant inbred-lines population of the cross Terese x K586. This cross was chosen because several mutants were obtained from cultivars Terese and Torsdag (K586 was derived from Torsdag). This collection of isogenic lines was used for the construction of F2 mapping populations in which polymorphic RAPD markers were already known and mapped. Moreover, the well-known problem in pea of variability in the linkage associations between crosses was avoided. This work contributes to the precise integration between the classical map and the molecular maps existing in pea.

Item Details

Item Type:Refereed Article
Research Division:Technology
Research Group:Medical Biotechnology
Research Field:Medical Biotechnology not elsewhere classified
Objective Division:Expanding Knowledge
Objective Group:Expanding Knowledge
Objective Field:Expanding Knowledge in the Environmental Sciences
Author:Batge, SL (Ms Shona Lee Batge)
Author:Murfet, IC (Professor Ian Murfet)
ID Code:14943
Year Published:1998
Web of Science® Times Cited:21
Deposited By:Plant Science
Deposited On:1998-08-01
Last Modified:2011-08-09
Downloads:0

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