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Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model

Citation

Thompson, JLM and West, DWD and Doering, TM and Budiono, BP and Lessard, SJ and Koch, LG and Britton, SL and Byrne, NM and Brown, MA and Ashton, KJ and Coffey, VG, Effect of short-term hindlimb immobilization on skeletal muscle atrophy and the transcriptome in a low compared with high responder to endurance training model, PLoS ONE, 17, (1) pp. e0261723. ISSN 1932-6203 (2022) [Refereed Article]


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Copyright 2022 Thompson et al. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1371/journal.pone.0261723

Abstract

Skeletal muscle atrophy is a physiological response to disuse, aging, and disease. We compared changes in muscle mass and the transcriptome profile after short-term immobilization in a divergent model of high and low responders to endurance training to identify biological processes associated with the early atrophy response. Female rats selectively bred for high response to endurance training (HRT) and low response to endurance training (LRT; n = 6/group; generation 19) underwent 3 day hindlimb cast immobilization to compare atrophy of plantaris and soleus muscles with line-matched controls (n = 6/group). RNA sequencing was utilized to identify Gene Ontology Biological Processes with differential gene set enrichment. Aerobic training performed prior to the intervention showed HRT improved running distance (+60.6 29.6%), while LRT were unchanged (-0.3 13.3%). Soleus atrophy was greater in LRT vs. HRT (-9.0 8.8 vs. 6.2 8.2%; P<0.05) and there was a similar trend in plantaris (-16.4 5.6% vs. -8.5 7.4%; P = 0.064). A total of 140 and 118 biological processes were differentially enriched in plantaris and soleus muscles, respectively. Soleus muscle exhibited divergent LRT and HRT responses in processes including autophagy and immune response. In plantaris, processes associated with protein ubiquitination, as well as the atrogenes (Trim63 and Fbxo32), were more positively enriched in LRT. Overall, LRT demonstrate exacerbated atrophy compared to HRT, associated with differential gene enrichments of biological processes. This indicates that genetic factors that result in divergent adaptations to endurance exercise, may also regulate biological processes associated with short-term muscle unloading.

Item Details

Item Type:Refereed Article
Research Division:Health Sciences
Research Group:Sports science and exercise
Research Field:Exercise physiology
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the health sciences
UTAS Author:Byrne, NM (Professor Nuala Byrne)
ID Code:149401
Year Published:2022
Deposited By:Health Sciences
Deposited On:2022-03-29
Last Modified:2022-04-13
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