eCite Digital Repository

Using portable X-ray fluorescence spectroscopy for inexpensive and quick determination of micronutrients in barley shoots


Khan, WA and Shabala, S and Cuin, TA and Zhou, M and Penrose, B, Using portable X-ray fluorescence spectroscopy for inexpensive and quick determination of micronutrients in barley shoots, Communications in Soil Science and Plant Analysis, 53, (11) pp. 1379-1384. ISSN 0010-3624 (2022) [Refereed Article]

PDF (Fully Published version)
Pending copyright assessment - Request a copy

Copyright Statement

Copyright 2022 Taylor & Francis Group, LLC

DOI: doi:10.1080/00103624.2022.2046033


Determination of elemental concentrations in plant tissue samples is critically important for analyzing their nutritional content. Micro-elemental analysis mostly relies on inductively coupled plasma (ICP) spectrometry techniques, which are expensive, time-consuming, laborious, and complicated. Portable X-ray fluorescence (pXRF) spectroscopy is widely applied in earth sciences but is rarely practiced to quantify elements in plant samples. In this pilot study, the reliability of pXRF results was compared with that of ICP-mass spectrometer (MS) to determine concentrations of four micronutrients zinc (Zn), iron (Fe), copper (Cu), and cadmium (Cd) in barley (Hordeum vulgare L.) shoot samples. Ground barley samples were scanned with the pXRF instrument. The concentrations of Zn recorded using this method correlated well with the ICP-MS results (R2 = 0.80), while Fe and Cu concentrations showed moderate correlations (R2 = 0.57 and 0.42, respectively). However, no significant correlation was found between ICP-MS- and pXRF-derived concentrations for Cd (p > .05). This work demonstrated the potential of pXRF as a reliable and cost-effective alternative to ICP methods for determining Zn in plant samples as it allows the analysis of a greater number of samples in a short period at considerably less expense.

Item Details

Item Type:Refereed Article
Keywords:barley, zinc, XRF, plant nutrition, biofortification, Hordeum vulgare, elements, pXRF, ICP-MS
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture nutrition
Objective Division:Plant Production and Plant Primary Products
Objective Group:Grains and seeds
Objective Field:Barley
UTAS Author:Khan, WA (Mr Waleed Khan)
UTAS Author:Shabala, S (Professor Sergey Shabala)
UTAS Author:Cuin, TA (Dr Tracey Cuin)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Penrose, B (Dr Beth Penrose)
ID Code:149020
Year Published:2022
Deposited By:Agriculture and Food Systems
Deposited On:2022-03-01
Last Modified:2022-12-08

Repository Staff Only: item control page