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Salinity effects on guard cell proteome in Chenopodium quinoa

Citation

Rasouli, F and Kiani-Pouya, A and Shabala, L and Li, L and Tahir, A and Yu, M and Hedrich, R and Chen, Z and Wilson, R and Zhang, H and Shabala, S, Salinity effects on guard cell proteome in Chenopodium quinoa, International Journal of Molecular Sciences, 22, (1) Article 428. ISSN 1422-0067 (2021) [Refereed Article]


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Copyright Statement

Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/).

DOI: doi:10.3390/ijms22010428

Abstract

Epidermal fragments enriched in guard cells (GCs) were isolated from the halophyte quinoa (Chenopodium quinoa Wild.) species, and the response at the proteome level was studied after salinity treatment of 300 mM NaCl for 3 weeks. In total, 2147 proteins were identified, of which 36% were differentially expressed in response to salinity stress in GCs. Up and downregulated proteins included signaling molecules, enzyme modulators, transcription factors and oxidoreductases. The most abundant proteins induced by salt treatment were desiccation-responsive protein 29B (50-fold), osmotin-like protein OSML13 (13-fold), polycystin-1, lipoxygenase, alpha-toxin, and triacylglycerol lipase (PLAT) domain-containing protein 3-like (eight-fold), and dehydrin early responsive to dehydration (ERD14) (eight-fold). Ten proteins related to the gene ontology term "response to ABA" were upregulated in quinoa GC; this included aspartic protease, phospholipase D and plastid-lipid-associated protein. Additionally, seven proteins in the sucrose-starch pathway were upregulated in the GC in response to salinity stress, and accumulation of tryptophan synthase and L-methionine synthase (enzymes involved in the amino acid biosynthesis) was observed. Exogenous application of sucrose and tryptophan, L-methionine resulted in reduction in stomatal aperture and conductance, which could be advantageous for plants under salt stress. Eight aspartic proteinase proteins were highly upregulated in GCs of quinoa, and exogenous application of pepstatin A (an inhibitor of aspartic proteinase) was accompanied by higher oxidative stress and extremely low stomatal aperture and conductance, suggesting a possible role of aspartic proteinase in mitigating oxidative stress induced by saline conditions.

Item Details

Item Type:Refereed Article
Keywords:quinoa, guard cell, stomata, salt stress, proteomics
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Food sciences
Research Field:Food sustainability
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Rasouli, F (Ms Fatemeh Rasouli)
UTAS Author:Kiani-Pouya, A (Dr Ali Kiani-Pouya)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:148963
Year Published:2021
Web of Science® Times Cited:7
Deposited By:Central Science Laboratory
Deposited On:2022-02-25
Last Modified:2022-03-15
Downloads:6 View Download Statistics

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