eCite Digital Repository

Proteomic investigation of brain, liver and intestine in high feed intake and low feed intake Chinook salmon (Oncorhynchus tshawytscha)

Citation

Esmaeili, M and Carter, CG and Wilson, R and Walker, SP and Miller, MR and Bridle, AR and Symonds, JE, Proteomic investigation of brain, liver and intestine in high feed intake and low feed intake Chinook salmon (Oncorhynchus tshawytscha), Aquaculture, 551 Article 737915. ISSN 0044-8486 (2022) [Refereed Article]

Copyright Statement

2022 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.aquaculture.2022.737915

Abstract

In groups of fish, there is often interindividual and intraindividual variation in feed intake. To better understand mechanisms that underpin feed intake, the proteomic profiles of brain, liver, and intestine in high feed intake (HFI) and low feed intake (LFI) Chinook salmon (Oncorhynchus tshawytscha) were investigated. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to quantify 2520 liver, 2783 intestine, and 4052 brain proteins in twenty-seven fish (12 HFI and 15 LFI individuals). Using a population of fish held in tanks, feed intake groups were selected from individual feed intake (%body weight/day (%BW/day)) measured in the meal prior to sampling (HFI: 1.01%BW/day, LFI: 0.41%BW/day). Growth rate and feed conversion ratio were not significantly different between HFI and LFI fish. Protein synthesis in both the intestine and liver of HFI was enriched, indicating increased cellular production in these tissues. In HFI fish, lipid metabolism was the most enriched pathway in the brain. Share of the meal (SM%), calculated as the share of the total feed supplied to the tank, was significantly higher in the HFI (1.590.25%) than the LFI (0.720.06%) group. This distribution of SM% may suggest that individuals in the HFI and LFI groups were dominant or subordinate, respectively. The LFI group may have suffered from internal or external stressors and accordingly, proteolysis and stress response were activated. This is the first study comparing individual fish with different measured feed intakes at the proteomic level and the outputs provide a preliminary insight into the fundamental molecular landscape of feed intake in Chinook salmon.

Item Details

Item Type:Refereed Article
Keywords:proteome, brain, liver, feed intake, bioinformatics, Chinook salmon, feeding hierarchies
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture and fisheries stock assessment
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Esmaeili, M (Mr Moha Esmaeili)
UTAS Author:Carter, CG (Professor Chris Carter)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Miller, MR (Dr Matthew Miller)
UTAS Author:Bridle, AR (Associate Professor Andrew Bridle)
ID Code:148958
Year Published:2022
Deposited By:Directorate
Deposited On:2022-02-25
Last Modified:2022-03-04
Downloads:0

Repository Staff Only: item control page