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Proteomic investigation of liver and white muscle in efficient and inefficient Chinook salmon (Oncorhynchus tshawytscha): fatty acid metabolism and protein turnover drive feed efficiency

Citation

Esmaeili, M and Carter, CG and Wilson, R and Walker, SP and Miller, MR and Bridle, A and Symonds, JE, Proteomic investigation of liver and white muscle in efficient and inefficient Chinook salmon (Oncorhynchus tshawytscha): fatty acid metabolism and protein turnover drive feed efficiency, Aquaculture, 542 Article 736855. ISSN 0044-8486 (2021) [Refereed Article]

Copyright Statement

2021 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.aquaculture.2021.736855

Abstract

Feed efficiency, the relative ability to convert feed nutrients into growth, is an important factor in the primary production of animals. Farming fish with improved feed efficiency is necessary to reduce production costs and achieve sustainability for the aquaculture industry. Proteomics provides an approach to discover biochemical mechanisms driving feed efficiency. To do this, we have evaluated the proteomic profile of liver and white muscle in efficient (EFF) and inefficient (INEFF) Chinook salmon (Oncorhynchus tshawytscha). Twenty-six fish, 14 EFF and 12 INEFF individuals, were assessed using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and a data-independent acquisition method. In total, 2433 liver and 635 white muscle proteins were quantified across all samples. Bioinformatics showed enrichment of gene ontology (GO) terms related to lipid metabolism in liver of EFF fish (~30% of total GO terms). Protein metabolism (~30% of total GO terms) were the top enriched pathways in white muscle of EFF fish. In INEFF fish, protein processing in endoplasmic reticulum and proteolysis (~40% of total GO terms) were the highest enriched GO terms in the liver. This is the first study to compare feed efficient and inefficient individuals at the proteomic level in an aquatic species and the results provide a preliminary insight into the fundamental molecular landscape of feed efficiency in Chinook salmon.

Item Details

Item Type:Refereed Article
Keywords:growth, growth efficiency, proteomics, genetic selection, chinook salmon, efficent fish
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Aquaculture
Objective Division:Animal Production and Animal Primary Products
Objective Group:Fisheries - aquaculture
Objective Field:Aquaculture fin fish (excl. tuna)
UTAS Author:Esmaeili, M (Mr Moha Esmaeili)
UTAS Author:Carter, CG (Professor Chris Carter)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Miller, MR (Dr Matthew Miller)
UTAS Author:Bridle, A (Associate Professor Andrew Bridle)
UTAS Author:Symonds, JE (Dr Jane Symonds)
ID Code:148957
Year Published:2021
Web of Science® Times Cited:9
Deposited By:Directorate
Deposited On:2022-02-25
Last Modified:2022-03-04
Downloads:0

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