148909 - Parathyroid carcinoma and adenoma co-existing in one patient.pdf (7.14 MB)
Parathyroid carcinoma and adenoma co-existing in one patient: case report and comparative proteomic analysis
journal contribution
posted on 2023-05-21, 05:52 authored by Ciregia, F, Cetani, F, Pardi, E, Soggiu, A, Piras, C, Zallocco, L, Borsari, S, Ronci, M, Vanni CarusoVanni Caruso, Marcocci, C, Mazzoni, MR, Lucacchini, A, Giusti, LBackground/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients and Methods: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN’s Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. Results: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. Conclusion: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.
History
Publication title
Cancer Genomics and ProteomicsVolume
18Issue
6Pagination
781-796ISSN
1109-6535Department/School
School of Pharmacy and PharmacologyPublisher
International Institute of Anticancer ResearchPlace of publication
GreeceRights statement
Copyright© 2021, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reservedRepository Status
- Open