Preview

PDF (Published version) 7Mb

Background/Aim: The lack of specific parathyroid carcinoma (PC) biomarkers in clinical practice points out the importance of analyzing the proteomic signature of this cancer. We performed a comparative proteomic analysis of PC and parathyroid adenoma (PA) co-existing in the same patient. Patients and Methods: PC and PA were taken from a 63-year-old patient. Using two-dimensional differential gel electrophoresis (2D-DIGE) coupled to mass spectrometry we examined the differences between PC and PA proteins. For validation, additional PC and PA samples were obtained from 10 patients. Western blot analysis was used to validate the difference of expression observed with 2D-DIGE analysis. Bioinfomatic analysis was performed using QIAGEN’s Ingenuity Pathways Analysis (IPA) to determine the predominant canonical pathways and interaction networks involved. Results: Thirty-three differentially expressed proteins were identified in PC compared to PA. Among these, ubiquitin C-terminal hydrolase-L1 (UCH-L1) was highly overexpressed in PC. The result was confirmed by Western Blot analysis in additional PC samples. Conclusion: Our comparative proteomic analysis of co-existing neoplasms allowed detecting specific and peculiar differences between PC and PA overcoming population biological variability.

Item Type:	Refereed Article
Keywords:	proteomics, purinergic system, drug discovery
Research Division:	Biomedical and Clinical Sciences
Research Group:	Pharmacology and pharmaceutical sciences
Research Field:	Pharmaceutical sciences
Objective Division:	Health
Objective Group:	Clinical health
Objective Field:	Treatment of human diseases and conditions
UTAS Author:	Caruso, V (Dr Vanni Caruso)
ID Code:	148909
Year Published:	2021
Web of Science® Times Cited:	2
Deposited By:	Pharmacy
Deposited On:	2022-02-18
Last Modified:	2022-03-04
Downloads:	7 View Download Statistics