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High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples

Citation

Faux, CE and McInnes, JC and Jarman, SN, High-throughput real-time PCR and melt curve analysis for sexing Southern Ocean seabirds using fecal samples, Theriogenology, 81, (6) pp. 870-874. ISSN 0093-691X (2014) [Refereed Article]


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Copyright Statement

Copyright 2014 The Authors. Published by Elsevier Inc. Open access under CC BY-NC-SA license. (https://creativecommons.org/licenses/by-nc-sa/3.0/)

DOI: doi:10.1016/j.theriogenology.2013.12.021

Abstract

Sex identification of birds is of great interest in ecological studies, however this can be very difficult in many species because their external features are almost monomorphic between the sexes. Molecular methodology has simplified this process but limitations still occur with widely accepted methods using polymerase chain reaction and gel electrophoresis, especially when applied to degraded DNA. Real-time polymerase chain reaction assays are emerging as a more efficient, sensitive, and higher throughput means of identification, but there are very few techniques validated using fecal samples and small target sizes. We present a real-time melt curve analysis assay targeting a small region of the CHD-1 gene allowing for high-throughput, sensitive, specific, and easy-to-interpret sexing results for a variety of Southern Ocean seabirds using fecal and tissue samples.

Item Details

Item Type:Refereed Article
Keywords:chromodomain helicase DNA-binding protein 1 gene, sexing, real-time polymerase chain reaction, avian sexing, feces, scat
Research Division:Biological Sciences
Research Group:Ecology
Research Field:Marine and estuarine ecology (incl. marine ichthyology)
Objective Division:Environmental Management
Objective Group:Marine systems and management
Objective Field:Marine biodiversity
UTAS Author:McInnes, JC (Dr Julie McInnes)
ID Code:148758
Year Published:2014
Web of Science® Times Cited:10
Deposited By:Ecology and Biodiversity
Deposited On:2022-02-07
Last Modified:2022-03-10
Downloads:2 View Download Statistics

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