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Evaluation of epigenetic age calculators between preeclampsia and normotensive pregnancies in an Australian cohort

Citation

Pruszkowska-Przybylska, P and Brennecke, S and Moses, EK and Melton, PE, Evaluation of epigenetic age calculators between preeclampsia and normotensive pregnancies in an Australian cohort, Scientific Reports, 12 Article 1664. ISSN 2045-2322 (2022) [Refereed Article]


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© The Author(s) 2022. This article is licensed under a Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. http://creat ivecommons.org/licenses/by/4.0/.

DOI: doi:10.1038/s41598-022-05744-4

Abstract

Advanced biological aging, as assessed through DNA methylation markers, is associated with several complex diseases. The associations between maternal DNA methylation age and preeclampsia (PE) have not been fully assessed. The aim of this study was to examine if increased maternal DNA methylation age (DNAmAge) was shown to be accelerated in women with PE when compared to women who had normotensive pregnancies. The case/control cohort available for study consisted of 166 women (89 with normotensive pregnancy, 77 with PE) recruited previously at the Royal Women’s Hospital in Melbourne, Australia. DNA methylation profiles were obtained using the Illumina EPIC Infinium array for analysis of genomic DNA isolated from whole blood. These profiles were used to calculate seven estimates of DNAmAge and included (1) Horvath, (2) Hannum, (3) Horvath Skin and Blood, (4) Wu, (5) PhenoAge, (6) telomere length and (7) GrimAge and its surrogate measures. Three measures of DNA methylation age acceleration were calculated for all seven measures using linear regression. Pearson's correlation was performed to investigate associations between chronological age and DNAmAge. Differences between chronological age and DNAmAge and epigenetic age acceleration were investigated using t-tests. No significant difference was observed for chronological age between women with PE (age = 30.53 ± 5.68) and women who had normotensive pregnancies (age = 31.76 ± 4.76). All seven DNAmAge measures were significantly correlated (p < 0.001) with chronological age. After accounting for multiple testing and investigating differences in DNAmAge between normotensive women and women with PE, only Wu DNAmAge was significant (p = 0.001). When examining differences for epigenetic age acceleration between PE and normotensive women Hannum, Wu, and PhenoAge DNAmAge estimates (p < 0.001) were significant for both epigenetic age acceleration and intrinsic acceleration models. We found that accelerated maternal DNAmAge is increased in women with PE in some models of epigenetic aging. This research underlines the importance for further investigation into the potential changes of differential DNA methylation in PE.

Item Details

Item Type:Refereed Article
Keywords:preeclampsia, DNA methylation, machine learning, epigenetic age, pregnancy
Research Division:Biomedical and Clinical Sciences
Research Group:Reproductive medicine
Research Field:Obstetrics and gynaecology
Objective Division:Health
Objective Group:Specific population health (excl. Indigenous health)
Objective Field:Women's and maternal health
UTAS Author:Moses, EK (Professor Eric Moses)
UTAS Author:Melton, PE (Dr Phillip Melton)
ID Code:148681
Year Published:2022
Funding Support:National Health and Medical Research Council (2001203)
Deposited By:Menzies Institute for Medical Research
Deposited On:2022-02-02
Last Modified:2022-03-03
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