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In planta transcriptome and proteome profiles of Spongospora subterranea in resistant and susceptible host environments illuminates regulatory principles underlying host-pathogen interaction

Citation

Balotf, S and Wilson, R and Tegg, RS and Nichols, DS and Wilson, CR, In planta transcriptome and proteome profiles of Spongospora subterranea in resistant and susceptible host environments illuminates regulatory principles underlying host-pathogen interaction, Biology, 10, (9) Article 840. ISSN 2079-7737 (2021) [Refereed Article]


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Copyright Statement

Copyright: 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/).

DOI: doi:10.3390/biology10090840

Abstract

Spongospora subterranea is an obligate biotrophic pathogen, causing substantial economic loss to potato industries globally. Currently, there are no fully effective management strategies for the control of potato diseases caused by S. subterranea. To further our understanding of S. subterranea biology during infection, we characterized the transcriptome and proteome of the pathogen during the invasion of roots of a susceptible and a resistant potato cultivar. A total of 7650 transcripts from S. subterranea were identified in the transcriptome analysis in which 1377 transcripts were differentially expressed between two cultivars. In proteome analysis, we identified 117 proteins with 42 proteins significantly changed in comparisons between resistant and susceptible cultivars. The functional annotation of transcriptome data indicated that the gene ontology terms related to the transportation and actin processes were induced in the resistant cultivar. The downregulation of enzyme activity and nucleic acid metabolism in the resistant cultivar suggests a probable influence of these processes in the virulence of S. subterranea. The protein analysis results indicated that the majority of differentially expressed proteins were related to the metabolic processes and transporter activity. The present study provides a comprehensive molecular insight into the multiple layers of gene regulation that contribute to S. subterranea infection and development in planta and illuminates the role of host immunity in affecting pathogen responses.

Item Details

Item Type:Refereed Article
Keywords:Spongospora subterranea, potato, in planta analysis, transcriptomics; proteomics
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Crop and pasture production
Research Field:Crop and pasture protection (incl. pests, diseases and weeds)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Field grown vegetable crops
UTAS Author:Balotf, S (Mr Sadegh Balotf)
UTAS Author:Wilson, R (Dr Richard Wilson)
UTAS Author:Tegg, RS (Dr Robert Tegg)
UTAS Author:Nichols, DS (Dr David Nichols)
UTAS Author:Wilson, CR (Professor Calum Wilson)
ID Code:148671
Year Published:2021
Funding Support:Australian Research Council (DP180103337)
Web of Science® Times Cited:3
Deposited By:TIA - Research Institute
Deposited On:2022-02-01
Last Modified:2022-03-07
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