eCite Digital Repository

A new cost-efficient technique for analysis of nectar sugars and dihydroxyacetone in Australian Leptospermum using liquid chromatography–tandem mass spectrometry


Wellington, CN and Nichols, DS and O'Grady, AP and Vaillancourt, RE and Potts, BM, A new cost-efficient technique for analysis of nectar sugars and dihydroxyacetone in Australian Leptospermum using liquid chromatography-tandem mass spectrometry, ACS Food Science & Technology, 1, (10) pp. 1928-1935. ISSN 2692-1944 (2021) [Refereed Article]

Copyright Statement

Copyright © 2021 American Chemical Society

DOI: doi:10.1021/acsfoodscitech.1c00253


The commercial value of Leptospermum honey increases relative to the nonperoxide bioactivity provided by the methylglyoxal concentration in the honey, which has been shown to correlate with dihydroxyacetone (DHA) concentration in the nectar from which the honey is derived. We detail a new, reliable method to simultaneously detect and quantify DHA, glucose, fructose, and sucrose levels in Leptospermum scoparium nectar using normal phase liquid chromatography–tandem mass spectrometry. Through use of an internal standard (ribose), and a 10-fold dilution of the nectar solution, repeatable calibration curves were achieved (R2 > 0.99). Precision was acceptable (<6%) for all analytes of interest and limits of detection ranged from 0.02 mg·L–1 (sucrose) to 1.43 mg·L–1 (glucose). The method was also effective at a 5-fold dilution and across analyte concentration ranges found naturally in L. scoparium nectar. Minimal sample preparation is required and a short analysis time of 6 min per sample is achieved, providing the Leptospermum honey industry with cost-effective and fast sample analysis.

Item Details

Item Type:Refereed Article
Keywords:medicinal honey, Leptospermum, nectar, ultraperformance liquid chromatography, mass-spectrometry, dihydroxyacetone, honey
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Horticultural production
Research Field:Horticultural crop improvement (incl. selection and breeding)
Objective Division:Plant Production and Plant Primary Products
Objective Group:Horticultural crops
Objective Field:Horticultural crops not elsewhere classified
UTAS Author:Wellington, CN (Mr Christopher Wellington)
UTAS Author:Nichols, DS (Dr David Nichols)
UTAS Author:O'Grady, AP (Dr Anthony O'Grady)
UTAS Author:Vaillancourt, RE (Professor Rene Vaillancourt)
UTAS Author:Potts, BM (Professor Brad Potts)
ID Code:148353
Year Published:2021
Deposited By:Plant Science
Deposited On:2022-01-02
Last Modified:2022-04-14

Repository Staff Only: item control page