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Utilizing RAFT polymerization for the preparation of well-defined bicontinuous porous polymeric supports: application to liquid chromatography separation of biomolecules

Citation

Khodabandeh, A and Arrua, RD and Thickett, SC and Hilder, EF, Utilizing RAFT polymerization for the preparation of well-defined bicontinuous porous polymeric supports: application to liquid chromatography separation of biomolecules, ACS Applied Materials and Interfaces, 13, (27) pp. 32075-32083. ISSN 1944-8244 (2021) [Refereed Article]


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DOI: doi:10.1021/acsami.1c03542

Abstract

Polymer-based monolithic high-performance liquid chromatography (HPLC) columns are normally obtained by conventional free-radical polymerization. Despite being straightforward, this approach has serious limitations with respect to controlling the structural homogeneity of the monolith. Herein, we explore a reversible addition–fragmentation chain transfer (RAFT) polymerization method for the fabrication of porous polymers with well-defined porous morphology and surface chemistry in a confined 200 μm internal diameter (ID) capillary format. This is achieved via the controlled polymerization-induced phase separation (controlled PIPS) synthesis of poly(styrene-co-divinylbenzene) in the presence of a RAFT agent dissolved in an organic solvent. The effects of the radical initiator/RAFT molar ratio as well as the nature and amount of the organic solvent were studied to target cross-linked porous polymers that were chemically bonded to the inner wall of a modified silica-fused capillary. The morphological and surface properties of the obtained polymers were thoroughly characterized by in situ nuclear magnetic resonance (NMR) experiments, nitrogen adsorption–desorption experiments, elemental analyses, field-emission scanning electron microscopy (FESEM), scanning electron microscopy-energy-dispersive X-ray (SEM-EDX) spectroscopy, and X-ray photoelectron spectroscopy (XPS) as well as time-of-flight secondary ion mass spectrometry (ToF-SIMS) revealing the physicochemical properties of these styrene-based materials. When compared with conventional synthetic methods, the controlled-PIPS approach affects the kinetics of polymerization by delaying the onset of phase separation, enabling the construction of materials with a smaller pore size. The results demonstrated the potential of the controlled-PIPS approach for the design of porous monolithic columns suitable for liquid separation of biomolecules such as peptides and proteins.

Item Details

Item Type:Refereed Article
Keywords:porous monoliths, cross-linked polymers, phase-separation, separation science, RAFT polymerization
Research Division:Chemical Sciences
Research Group:Macromolecular and materials chemistry
Research Field:Macromolecular materials
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Thickett, SC (Dr Stuart Thickett)
ID Code:147681
Year Published:2021
Web of Science® Times Cited:3
Deposited By:Chemistry
Deposited On:2021-11-11
Last Modified:2022-05-02
Downloads:0

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