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Calcium-dependent hydrogen peroxide mediates hydrogen-rich water-reduced cadmium uptake in plant roots

Citation

Wu, Q and Huang, L and Su, N and Shabala, L and Wang, H and Huang, X and Wen, R and Yu, M and Cui, J and Shabala, S, Calcium-dependent hydrogen peroxide mediates hydrogen-rich water-reduced cadmium uptake in plant roots, Plant Physiology, 183, (3) pp. 1331-1344. ISSN 0032-0889 (2020) [Refereed Article]

Copyright Statement

Copyright 2020 American Society of Plant Biologists

DOI: doi:10.1104/pp.20.00377

Abstract

Hydrogen gas (H2) has a possible signaling role in many developmental and adaptive plant responses, including mitigating the harmful effects of cadmium (Cd) uptake from soil. We used electrophysiological and molecular approaches to understand how H2 ameliorates Cd toxicity in pak choi (Brassica campestris ssp. chinensis). Exposure of pak choi roots to Cd resulted in a rapid increase in the intracellular H2 production. Exogenous application of hydrogen-rich water (HRW) resulted in a Cd-tolerant phenotype, with reduced net Cd uptake and accumulation. We showed that this is dependent upon the transport of calcium ions (Ca2+) across the plasma membrane and apoplastic generation of hydrogen peroxide (H2O2) by respiratory burst oxidase homolog (BcRbohD). The reduction in root Cd uptake was associated with the application of exogenous HRW or H2O2 This reduction was abolished in the iron-regulated transporter1 (Atirt1) mutant of Arabidopsis (Arabidopsis thaliana), and pak choi pretreated with HRW showed decreased BcIRT1 transcript levels. Roots exposed to HRW had rapid Ca2+ influx, and Cd-induced Ca2+ leakage was alleviated. Two Ca2+ channel blockers, gadolinium ion (Gd3+) and lanthanum ion (La3+), eliminated the HRW-induced increase in BcRbohD expression, H2O2 production, and Cd2+ influx inhibition. Collectively, our results suggest that the Cd-protective effect of H2 in plants may be explained by its control of the plasma membrane-based NADPH oxidase encoded by RbohD, which operates upstream of IRT1 and regulates root Cd uptake at both the transcriptional and functional levels. These findings provide a mechanistic explanation for the alleviatory role of H2 in Cd accumulation and toxicity in plants.

Item Details

Item Type:Refereed Article
Keywords:roots, hydrogen-rich water, cadmium uptake, calcium
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Wu, Q (Mr Qi Wu)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Wang, H (Miss Haiyang Wang)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:147651
Year Published:2020
Web of Science® Times Cited:17
Deposited By:TIA - Research Institute
Deposited On:2021-11-10
Last Modified:2022-04-14
Downloads:0

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