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Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots
Citation
Alnayef, M and Solis, C and Shabala, L and Ogura, T and Chen, Z and Bose, J and Maathuis, FJM and Venkataraman, G and Tanoi, K and Yu, M and Zhou, M and Horie, T and Shabala, S, Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots, International Journal of Molecular Sciences, 21, (14) Article 4882. ISSN 1422-0067 (2020) [Refereed Article]
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Copyright Statement
Copyright 2020 The Author(s) Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/
Abstract
In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance. This gene is harbored by the SKC1 locus, and its role was attributed to Na+ unloading from the xylem. No direct evidence, however, was provided in previous studies. Also, the reported function of SKC1 on the loading and delivery of K+ to the shoot remains to be explained. In this work, we used an electrophysiological approach to compare the kinetics of Na+ uptake by root xylem parenchyma cells using wild type (WT) and NIL(SKC1) plants. Our data showed that Na+ reabsorption was observed in WT, but not NIL(SKC1) plants, thus questioning the functional role of HKT1;5 as a transporter operating in the direct Na+ removal from the xylem. Instead, changes in the expression level of HKT1;5 altered the activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in the rice epidermis and stele, explaining the observed phenotype. We conclude that the role of HKT1;5 in plant salinity tolerance cannot be attributed to merely reducing Na+ concentration in the xylem sap but triggers a complex feedback regulation of activities of other transporters involved in the maintenance of plant ionic homeostasis and signaling under stress conditions.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | rice roots, salinity, membrane transporters, OsHKT1;5, K+, Ca2+, salinity stress, xylem loading, sodium, potassium, SKC1, HAK5, GORK, RBOHD, epidermis, stele |
| Research Division: | Biological Sciences |
| Research Group: | Plant biology |
| Research Field: | Plant physiology |
| Objective Division: | Plant Production and Plant Primary Products |
| Objective Group: | Other plant production and plant primary products |
| Objective Field: | Other plant production and plant primary products not elsewhere classified |
| UTAS Author: | Alnayef, M (Mr Mohammad Alnayef) |
| UTAS Author: | Solis, C (Ms Celymar Solis) |
| UTAS Author: | Shabala, L (Associate Professor Lana Shabala) |
| UTAS Author: | Ogura, T (Mr Takaaki Ogura) |
| UTAS Author: | Bose, J (Dr Jayakumar Bose) |
| UTAS Author: | Zhou, M (Professor Meixue Zhou) |
| UTAS Author: | Shabala, S (Professor Sergey Shabala) |
| ID Code: | 147650 |
| Year Published: | 2020 |
| Web of Science® Times Cited: | 19 |
| Deposited By: | TIA - Research Institute |
| Deposited On: | 2021-11-10 |
| Last Modified: | 2022-04-22 |
| Downloads: | 5 View Download Statistics |
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