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Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots

Citation

Alnayef, M and Solis, C and Shabala, L and Ogura, T and Chen, Z and Bose, J and Maathuis, FJM and Venkataraman, G and Tanoi, K and Yu, M and Zhou, M and Horie, T and Shabala, S, Changes in expression level of OsHKT1;5 alters activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in salinized rice roots, International Journal of Molecular Sciences, 21, (14) Article 4882. ISSN 1422-0067 (2020) [Refereed Article]


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Copyright 2020 The Author(s) Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.3390/ijms21144882

Abstract

In rice, the OsHKT1;5 gene has been reported to be a critical determinant of salt tolerance. This gene is harbored by the SKC1 locus, and its role was attributed to Na+ unloading from the xylem. No direct evidence, however, was provided in previous studies. Also, the reported function of SKC1 on the loading and delivery of K+ to the shoot remains to be explained. In this work, we used an electrophysiological approach to compare the kinetics of Na+ uptake by root xylem parenchyma cells using wild type (WT) and NIL(SKC1) plants. Our data showed that Na+ reabsorption was observed in WT, but not NIL(SKC1) plants, thus questioning the functional role of HKT1;5 as a transporter operating in the direct Na+ removal from the xylem. Instead, changes in the expression level of HKT1;5 altered the activity of membrane transporters involved in K+ and Ca2+ acquisition and homeostasis in the rice epidermis and stele, explaining the observed phenotype. We conclude that the role of HKT1;5 in plant salinity tolerance cannot be attributed to merely reducing Na+ concentration in the xylem sap but triggers a complex feedback regulation of activities of other transporters involved in the maintenance of plant ionic homeostasis and signaling under stress conditions.

Item Details

Item Type:Refereed Article
Keywords:rice roots, salinity, membrane transporters, OsHKT1;5, K+, Ca2+, salinity stress, xylem loading, sodium, potassium, SKC1, HAK5, GORK, RBOHD, epidermis, stele
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Alnayef, M (Mr Mohammad Alnayef)
UTAS Author:Solis, C (Ms Celymar Solis)
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Ogura, T (Mr Takaaki Ogura)
UTAS Author:Bose, J (Dr Jayakumar Bose)
UTAS Author:Zhou, M (Professor Meixue Zhou)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:147650
Year Published:2020
Web of Science® Times Cited:11
Deposited By:TIA - Research Institute
Deposited On:2021-11-10
Last Modified:2022-04-22
Downloads:0

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