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Lipid kinases PIP5K7 and PIP5K9 are required for polyamine-triggered K+ efflux in Arabidopsis roots

Citation

Zarza, X and Van Wijk, R and Shabala, L and Hunkeler, A and Lefebvre, M and Rodriguez-Villalon, A and Shabala, S and Tiburcio, AF and Heilmann, I and Munnik, T, Lipid kinases PIP5K7 and PIP5K9 are required for polyamine-triggered K+ efflux in Arabidopsis roots, The Plant Journal, 104, (2) pp. 416-432. ISSN 1365-313X (2020) [Refereed Article]


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Copyright Statement

© 2020 The Authors. The Plant Journal published by Society for Experimental Biology and John Wiley & Sons Ltd This is an open access article under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, (https://creativecommons.org/licenses/by/4.0/) which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

DOI: doi:10.1111/tpj.14932

Abstract

Polyamines, such as putrescine, spermidine and spermine (Spm), are low-molecular-weight polycationic molecules present in all living organisms. Despite their implication in plant cellular processes, little is known about their molecular mode of action. Here, we demonstrate that polyamines trigger a rapid increase in the regulatory membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), and that this increase is required for polyamine effects on K+ efflux in Arabidopsis roots. Using in vivo 32Pi-labelling of Arabidopsis seedlings, low physiological (μm) concentrations of Spm were found to promote a rapid PIP2 increase in roots that was time- and dose-dependent. Confocal imaging of a genetically encoded PIP2 biosensor revealed that this increase was triggered at the plasma membrane. Differential 32Pi-labelling suggested that the increase in PIP2 was generated through activation of phosphatidylinositol 4-phosphate 5-kinase (PIP5K) activity rather than inhibition of a phospholipase C or PIP2 5-phosphatase activity. Systematic analysis of transfer DNA insertion mutants identified PIP5K7 and PIP5K9 as the main candidates involved in the Spm-induced PIP2 response. Using non-invasive microelectrode ion flux estimation, we discovered that the Spm-triggered K+ efflux response was strongly reduced in pip5k7 pip5k9 seedlings. Together, our results provide biochemical and genetic evidence for a physiological role of PIP2 in polyamine-mediated signalling controlling K+ flux in plants.

Item Details

Item Type:Refereed Article
Keywords:Arabidopsis, roots, K+ flux, polyamine, lipid kinases, PIP5K7, PIP5K9, phosphoinositide signalling, phosphatidylinositol 4,5-bisphosphate (PIP2), phosphatidylinositol 4-phosphate 5-kinase (PIP5K), phosphatidic acid (PA), phospholipids
Research Division:Biological Sciences
Research Group:Plant biology
Research Field:Plant physiology
Objective Division:Plant Production and Plant Primary Products
Objective Group:Other plant production and plant primary products
Objective Field:Other plant production and plant primary products not elsewhere classified
UTAS Author:Shabala, L (Associate Professor Lana Shabala)
UTAS Author:Shabala, S (Professor Sergey Shabala)
ID Code:147647
Year Published:2020
Web of Science® Times Cited:19
Deposited By:TIA - Research Institute
Deposited On:2021-11-10
Last Modified:2022-04-21
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