Revealing the role of the calcineurin B-like protein-interacting protein kinase 9 (CIPK9) in rice adaptive responses to salinity, osmotic stress, and K+ deficiency

In plants, calcineurin B-like (CBL) proteins and their interacting protein kinases (CIPK) form functional complexes that transduce downstream signals to membrane effectors assisting in their adaptation to adverse environmental conditions. This study addresses the issue of the physiological role of CIPK9 in adaptive responses to salinity, osmotic stress, and K⁺ deficiency in rice plants. Whole-plant physiological studies revealed that Oscipk9 rice mutant lacks a functional CIPK9 gene and displayed a mildly stronger phenotype, both under saline and osmotic stress conditions. The reported difference was attributed to the ability of Oscipk9 to maintain significantly higher stomatal conductance (thus, a greater carbon gain). Oscipk9 plants contained much less K⁺ in their tissues, implying the role of CIPK9 in K⁺ acquisition and homeostasis in rice. Oscipk9 roots also showed hypersensitivity to ROS under conditions of low K⁺ availability suggesting an important role of H₂O₂ signalling as a component of plant adaptive responses to a low-K environment. The likely mechanistic basis of above physiological responses is discussed.