University of Tasmania
Browse

File(s) under permanent embargo

Cell surface and intracellular auxin signalling for H+ fluxes in root growth

journal contribution
posted on 2023-05-21, 03:58 authored by Li, L, Verstraeten, I, Roosjen, M, Takahashi, K, Rodriguez, L, Merrin, J, Chen, J, Svetlana ShabalaSvetlana Shabala, Smet, W, Ren, H, Vanneste, S, Sergey ShabalaSergey Shabala, De Rybel, B, Weijers, D, Kinoshita, T, Gray, WM, Friml, J
Growth regulation tailors development in plants to their environment. A prominent example of this is the response to gravity, in which shoots bend up and roots bend down1. This paradox is based on opposite effects of the phytohormone auxin, which promotes cell expansion in shoots while inhibiting it in roots via a yet unknown cellular mechanism2. Here, by combining microfluidics, live imaging, genetic engineering and phosphoproteomics in Arabidopsis thaliana, we advance understanding of how auxin inhibits root growth. We show that auxin activates two distinct, antagonistically acting signalling pathways that converge on rapid regulation of apoplastic pH, a causative determinant of growth. Cell surface-based TRANSMEMBRANE KINASE1 (TMK1) interacts with and mediates phosphorylation and activation of plasma membrane H+-ATPases for apoplast acidification, while intracellular canonical auxin signalling promotes net cellular H+ influx, causing apoplast alkalinization. Simultaneous activation of these two counteracting mechanisms poises roots for rapid, fine-tuned growth modulation in navigating complex soil environments.

History

Publication title

Nature

Volume

599

Pagination

273-277

ISSN

0028-0836

Department/School

Tasmanian Institute of Agriculture (TIA)

Publisher

Nature Publishing Group

Place of publication

Macmillan Building, 4 Crinan St, London, England, N1 9Xw

Rights statement

Copyright 2021 Nature

Repository Status

  • Restricted

Socio-economic Objectives

Other plant production and plant primary products not elsewhere classified

Usage metrics

    University Of Tasmania

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC