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Myelin Protein Zero180–199 Peptide Induced Experimental Autoimmune Neuritis in C57BL/6 Mice
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posted on 2023-05-22, 19:28 authored by Gonsalvez, DG, Yoo, SW, Craig, GA, Wood, RJ, Jessica FletcherJessica Fletcher, Murray, SS, Xiao, JMouse models of peripheral demyelinating neuropathy play an important role in enabling the study of disease pathogenesis. Further, induction in transgenic mice allows for the precise interrogation of disease mechanisms, as well as the analysis of the efficacy and mechanisms of potential new therapies. Here we describe a method to successfully induce experimental autoimmune neuritis (EAN) using myelin protein zero (P0)180–199 peptide in combination with Freund’s complete adjuvant and pertussis toxin in the C57BL/6 mouse strain. We also outline a sensitive paradigm of accurately assessing the extent of functional deficits occurring in murine EAN.
History
Publication title
MyelinEditors
A WoodhooPagination
243-250ISBN
978-1-4939-7861-8Department/School
Menzies Institute for Medical ResearchPublisher
Humana PressPlace of publication
United StatesExtent
23Rights statement
Copyright 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018Repository Status
- Restricted