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Absence of TNF leads to alternative activation in peritoneal macrophages in experimental listeria monocytogenes infection
Citation
Li, X and Chen, C and Zhang, L and Cheng, X and Geng, H and Ji, Q and Li, C and Chen, H and Korner, H and Liu, X, Absence of TNF leads to alternative activation in peritoneal macrophages in experimental listeria monocytogenes infection, Immunological Investigations pp. 1-18. ISSN 0882-0139 (2021) [Refereed Article]
Copyright Statement
Copyright 2021 Taylor & Francis Group
DOI: doi:10.1080/08820139.2021.1902346
Abstract
Macrophages are crucial effectors of innate immunity against the pathogenic bacterium Listeria monocytogenes. The pro-inflammatory cytokine tumour necrosis factor-α (TNF) has been shown to be crucial for resistance to L. monocytogenes and mice deficient in TNF signalling succumb quickly after infection. However, the mechanisms underlying TNF-mediated defence against L. monocytogenes infection have not been completely elucidated. Here, we demonstrate that TNF concurrently functions to support a pro-inflammatory M1 phenotype while actively blocking macrophage polarization to the M2 phenotype. Compared to WT mice, peritoneal macrophages in TNF-deficient mice inoculated with L. monocytogenes respond with M2 polarization by upregulating Arg1. Consistently, TNF blockade in vitro resulted in M2 polarization in peritoneal macrophages during L. monocytogenes infection. Additionally, TNF promotes the transition from M2 to M1 polarization in peritoneal macrophages. Further investigation of peritoneal macrophage polarization suggested the NF-κB pathway is involved in the TNF-dependent M2 to M1 shift. Conversely, treatment of peritoneal macrophage with a PPARγ agonist blunted the expression of M1 genes induced by TNF and reduced NF-κB signalling pathway activation. Competing signalling mechanisms therefore play an essential role in the ability of peritoneal macrophage to resolve L. monocytogenes infections with TNF playing an essential role in driving M1 polarization.
Item Details
| Item Type: | Refereed Article |
|---|---|
| Keywords: | Listeria monocytogenes, PPARγ, TNF, macrophage polarization |
| Research Division: | Biomedical and Clinical Sciences |
| Research Group: | Immunology |
| Research Field: | Innate immunity |
| Objective Division: | Health |
| Objective Group: | Clinical health |
| Objective Field: | Diagnosis of human diseases and conditions |
| UTAS Author: | Li, X (Ms Amy Li) |
| UTAS Author: | Korner, H (Professor Heinrich Korner) |
| ID Code: | 147112 |
| Year Published: | 2021 |
| Web of Science® Times Cited: | 2 |
| Deposited By: | Menzies Institute for Medical Research |
| Deposited On: | 2021-10-13 |
| Last Modified: | 2022-08-25 |
| Downloads: | 0 |
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