eCite Digital Repository

Efficacy of estradiol in feminising the eastern mosquitofish, Gambusia holbrooki: advance towards developing a genetic control option

Citation

Norazmi-Lokman, NH and Purser, GJ and Patil, JG, Efficacy of estradiol in feminising the eastern mosquitofish, Gambusia holbrooki: advance towards developing a genetic control option, Marine and Freshwater Research, 72, (11) pp. 1657-1666. ISSN 1323-1650 (2021) [Refereed Article]

Copyright Statement

Journal compilation copyright CSIRO 2021

DOI: doi:10.1071/MF21104

Abstract

As a first step towards developing genetic option to control pest populations of Gambusia holbrooki, this study investigated the efficacy of oestradiol (E2) to feminise the species. The oestrogen was administered orally via food to neonates (Experiment 1) or embryos through gravid females (Experiment 2) at dosage between 50 and 400 mg kg–1 of feed. Two control groups consisted of (C1) normal feed and (C2) feed mixed with 70% ethanol. In Experiment 1, 100% feminisation was observed at all E2 concentrations except at 400 mg kg–1 where no treated individuals survived. There was a significantly (P < 0.05) lower mean survival rate (MSR) of all E2-treated juveniles than of controls (C1: 71.73 ± 22.86%; C2: 70.02 ± 18.26%), with 50 mg kg–1 showing the best MSR (66.38 ± 12.34%). In Experiment 2, 100% feminisation was achieved at E2 concentrations of 200–400 mg kg–1 food, with the best MSR (59.33 ± 12.54%) at 200 mg kg–1 concentration, which was, however, significantly lower than in control groups (C1: 79.96 ± 20.33%; C2: 77.09 ± 10.32%). The reproductive output and gestation period of gravid females were not affected by E2 exposure. The outcomes provide a framework for reliable sex reversal (feminisation) in this fish, paving way for developing genetic strategies to manage and eradicate this pest fish.

Item Details

Item Type:Refereed Article
Keywords:neonate, invasive fish, livebearer, endocrine disrupting compound, sex reversal
Research Division:Agricultural, Veterinary and Food Sciences
Research Group:Fisheries sciences
Research Field:Fish pests and diseases
Objective Division:Environmental Management
Objective Group:Fresh, ground and surface water systems and management
Objective Field:Control of pests, diseases and exotic species in fresh, ground and surface water
UTAS Author:Norazmi-Lokman, NH (Dr Lokman Norazmi)
UTAS Author:Purser, GJ (Associate Professor John Purser)
UTAS Author:Patil, JG (Dr Jawahar PATIL)
ID Code:146730
Year Published:2021
Deposited By:Sustainable Marine Research Collaboration
Deposited On:2021-09-23
Last Modified:2022-01-07
Downloads:0

Repository Staff Only: item control page