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Systems serology detects functionally distinct coronavirus antibody features in children and elderly

Citation

Selva, KJ and van de Sandt, CE and Lemke, MM and Lee, CY and Shoffner, SK and Chua, BY and Davis, SK and Nguyen, THO and Rowntree, LC and Hensen, L and Koutsakos, M and Wong, CY and Mordant, F and Jackson, DC and Flanagan, KL and Crowe, J and Tosif, S and Neeland, MR and Sutton, P and Licciardi, PV and Crawford, NW and Cheng, AC and Doolan, DL and Amanat, F and Krammer, F and Chappell, K and Modhiran, N and Watterson, D and Young, P and Lee, WS and Wines, BD and Hogarth, PM and Esterbauer, R and Kelly, HG and Tan, HX and Juno, JA and Wheatley, AK and Kent, SJ and Arnold, KB and Kedzierska, K and Chung, AW, Systems serology detects functionally distinct coronavirus antibody features in children and elderly, Nature Communications, 12, (1) ISSN 2041-1723 (2021) [Refereed Article]


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Copyright 2021 The Authors. Licensed under Creative Commons Attribution 4.0 International (CC BY 4.0) https://creativecommons.org/licenses/by/4.0/

DOI: doi:10.1038/s41467-021-22236-7

Abstract

The hallmarks of COVID-19 are higher pathogenicity and mortality in the elderly compared to children. Examining baseline SARS-CoV-2 cross-reactive immunological responses, induced by circulating human coronaviruses (hCoVs), is needed to understand such divergent clinical outcomes. Here we show analysis of coronavirus antibody responses of pre-pandemic healthy children (n = 89), adults (n = 98), elderly (n = 57), and COVID-19 patients (n = 50) by systems serology. Moderate levels of cross-reactive, but non-neutralizing, SARS-CoV-2 antibodies are detected in pre-pandemic healthy individuals. SARS-CoV-2 antigen-specific Fcγ receptor binding accurately distinguishes COVID-19 patients from healthy individuals, suggesting that SARS-CoV-2 infection induces qualitative changes to antibody Fc, enhancing Fcγ receptor engagement. Higher cross-reactive SARS-CoV-2 IgA and IgG are observed in healthy elderly, while healthy children display elevated SARS-CoV-2 IgM, suggesting that children have fewer hCoV exposures, resulting in less-experienced but more polyreactive humoral immunity. Age-dependent analysis of COVID-19 patients, confirms elevated class-switched antibodies in elderly, while children have stronger Fc responses which we demonstrate are functionally different. These insights will inform COVID-19 vaccination strategies, improved serological diagnostics and therapeutics.

Item Details

Item Type:Refereed Article
Research Division:Biological Sciences
Research Group:Microbiology
Research Field:Virology
Objective Division:Health
Objective Group:Clinical health
Objective Field:Treatment of human diseases and conditions
UTAS Author:Flanagan, KL (Dr Katie Flanagan)
ID Code:146647
Year Published:2021
Web of Science® Times Cited:32
Deposited By:Health Sciences
Deposited On:2021-09-20
Last Modified:2021-10-28
Downloads:4 View Download Statistics

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