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Thread-based isotachophoresis coupled with desorption electrospray ionization mass spectrometry for clean-up, preconcentration, and determination of alkaloids in biological fluids

Citation

Chen, L and Ghiasvand, A and Lam, SC and Sanz Rodriguez, ES and Innes, PC and Paull, B, Thread-based isotachophoresis coupled with desorption electrospray ionization mass spectrometry for clean-up, preconcentration, and determination of alkaloids in biological fluids, Analytica Chimica Acta Article 338810. ISSN 0003-2670 (In Press) [Refereed Article]

Copyright Statement

2021 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.aca.2021.338810

Abstract

A thread-based isotachophoresis method coupled with desorption electrospray ionization mass spectrometry (TB-ITP-DESI-MS) was developed and applied for clean-up, preconcentration, and determination of alkaloids in biological fluids. This simple approach enables the focusing and rapid analysis of analytes of interest in complex matrices that are otherwise challenging using direct ambient mass spectrometry. The TB-ITP platform components were rapidly and reproducibly fabricated at low-cost using 3D printing. A single string of nylon 6 thread was used as the electrophoresis substrate and a cotton knot, tied to the nylon thread, was used as the trapping zone of the ITP focused model analytes (coptisine, berberine and palmatine). The trapping efficiency was evaluated upon different commercially available threads with different chemical properties and cotton was selected as the best material due to its highest trapping efficiency and subsequent DESI-MS ionization efficiency. Up to 11.6-fold increase in signal to noise ratio (S/N) was obtained using the proposed method compared to direct DESI-MS detection, due to the reduced matrix interference and focusing. The results demonstrated that the TBITP- DESI-MS approach is a viable solution for the analysis of complicated biological fluid samples.

Item Details

Item Type:Refereed Article
Keywords:Thread-based isotachophoresis, desorption electrospray ionization mass spectrometry, alkaloids, biological fluids
Research Division:Chemical Sciences
Research Group:Analytical chemistry
Research Field:Separation science
Objective Division:Expanding Knowledge
Objective Group:Expanding knowledge
Objective Field:Expanding knowledge in the chemical sciences
UTAS Author:Chen, L (Miss Liang Chen)
UTAS Author:Ghiasvand, A (Professor Alireza Ghiasvand)
UTAS Author:Lam, SC (Mr Shing Chung Lam)
UTAS Author:Sanz Rodriguez, ES (Dr Estrella Sanz Rodriguez)
UTAS Author:Paull, B (Professor Brett Paull)
ID Code:146507
Year Published:In Press
Deposited By:Chemistry
Deposited On:2021-09-09
Last Modified:2021-12-01
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