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A fast and simple method for determination of β-carotene in commercial fruit juice by cloud point extraction-cold column trapping combined with UV-Vis spectrophotometry

journal contribution
posted on 2023-05-21, 02:20 authored by Safdarian, M, Hashemi, P, Alireza GhiasvandAlireza Ghiasvand
Cloud point extraction with cold column trapping (CPE-CCT) was used for the rapid preconcentration and UV-Vis spectroscopy of beta-carotene in fruit juice samples. A central composite design was employed to optimize parameters such as pH, incubation time, cloud point temperature and surfactant concentration. A detection limit of 0.01 mg/L of beta-carotene (3SB/m), a coefficient of determination of 0.998 and a linear range of 0.04-10 mg/L were obtained. The CPE-CCT method was confirmed in comparison with the corresponding direct HPLC standard method. A simple, portable and cost-effective device was also utilized. Owing to eliminating centrifugation, the conditions of CPE-CCT were more moderate and its sample handling easier compared to conventional CPE.

History

Publication title

Food Chemistry

Volume

343

Article number

128481

Number

128481

Pagination

1-7

ISSN

0308-8146

Department/School

School of Natural Sciences

Publisher

Elsevier Sci Ltd

Place of publication

The Boulevard, Langford Lane, Kidlington, Oxford, England, Oxon, Ox5 1Gb

Rights statement

© 2020 Elsevier Ltd.

Repository Status

  • Restricted

Socio-economic Objectives

Other plant production and plant primary products not elsewhere classified

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