Cloud point extraction with cold column trapping (CPE-CCT) was used for the rapid preconcentration and UV-Vis spectroscopy of beta-carotene in fruit juice samples. A central composite design was employed to optimize parameters such as pH, incubation time, cloud point temperature and surfactant concentration. A detection limit of 0.01 mg/L of beta-carotene (3SB/m), a coefficient of determination of 0.998 and a linear range of 0.04-10 mg/L were obtained. The CPE-CCT method was confirmed in comparison with the corresponding direct HPLC standard method. A simple, portable and cost-effective device was also utilized. Owing to eliminating centrifugation, the conditions of CPE-CCT were more moderate and its sample handling easier compared to conventional CPE.

Item Type:	Refereed Article
Keywords:	cloud point extraction, cold column trapping, multivariate optimization, beta-carotene
Research Division:	Chemical Sciences
Research Group:	Analytical chemistry
Research Field:	Separation science
Objective Division:	Plant Production and Plant Primary Products
Objective Group:	Other plant production and plant primary products
Objective Field:	Other plant production and plant primary products not elsewhere classified
UTAS Author:	Ghiasvand, A (Professor Alireza Ghiasvand)
ID Code:	146500
Year Published:	2021
Web of Science® Times Cited:	2
Deposited By:	Chemistry
Deposited On:	2021-09-09
Last Modified:	2021-11-16
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