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Ancient DNA from marine sediments: precautions and considerations for seafloor coring, sample handling and data generation


Armbrecht, LH and Coolen, MJL and Lejzerowicz, F and George, SC and Negandhi, K and Suzuki, Y and Young, J and Foster, NR and Armand, LK and Cooper, A and Ostrowski, M and Focardi, A and Stat, M and Moreau, JW and Weyrich, LS, Ancient DNA from marine sediments: precautions and considerations for seafloor coring, sample handling and data generation, Earth Science Reviews, 196 Article 102887. ISSN 0012-8252 (2019) [Refereed Article]

Copyright Statement

2019 Elsevier B.V. All rights reserved.

DOI: doi:10.1016/j.earscirev.2019.102887


The study of ancient DNA (aDNA) from sediments (sedaDNA) offers great potential for paleoclimate interpretation, and has recently been applied as a tool to characterise past marine life and environments from deep ocean sediments over geological timescales. Using sedaDNA, palaeo-communities have been detected, including prokaryotes and eukaryotes that do not fossilise, thereby revolutionising the scope of marine micropalaeontological research. However, many studies to date have not reported on the measures taken to prove the authenticity of sedaDNA-derived data from which conclusions are drawn. aDNA is highly fragmented and degraded and extremely sensitive to contamination by non-target environmental DNA. Contamination risks are particularly high on research vessels, drilling ships and platforms, where logistics and facilities do not yet allow for sterile sediment coring, and due consideration needs to be given to sample processing and analysis following aDNA guidelines. This review clarifies the use of aDNA terminology, discusses common pitfalls and highlights the urgency behind adopting new standards for marine sedaDNA research, with a focus on sampling optimisation to facilitate the incorporation of routine sedaDNA research into International Ocean Discovery Program (IODP) operations. Currently available installations aboard drilling ships and platforms are reviewed, improvements suggested, analytical approaches detailed, and the controls and documentation necessary to support the authenticity of aDNA retrieved from deep-sea sediment cores is outlined. Beyond practical considerations, concepts relevant to the study of past marine biodiversity based on sedaDNA, and the applicability of the new guidelines to the study of other contamination-susceptible environments (permafrost and outer space) are discussed.

Item Details

Item Type:Refereed Article
Keywords:sedimentary ancient DNA, scientific drilling, IODP, field work, phytoplankton, metagenomics, authenticity
Research Division:Biological Sciences
Research Group:Genetics
Research Field:Genomics
Objective Division:Environmental Management
Objective Group:Marine systems and management
Objective Field:Marine biodiversity
UTAS Author:Armbrecht, LH (Dr Linda Armbrecht)
ID Code:146469
Year Published:2019
Web of Science® Times Cited:23
Deposited By:Ecology and Biodiversity
Deposited On:2021-09-08
Last Modified:2021-11-19

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