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Targeting histone acetylation dynamics and oncogenic transcription by catalytic P300/CBP inhibition
journal contribution
posted on 2023-05-21, 02:15 authored by Hogg, SJ, Motorna, O, Cluse, LA, Johanson, TM, Coughlan, HD, Raviram, R, Myers, RM, Costacurta, M, Todorovski, I, Pijpers, L, Bjelosevic, S, Williams, T, Shannon HuskinsShannon Huskins, Kearney, CJ, Devlin, JR, Fan, Z, Jabbari, JS, Martin, BP, Fareh, M, Kelly, MJ, Dupere-Richer, D, Sandow, JJ, Feran, B, Knight, D, Khong, T, Spencer, A, Harrison, SJ, Gregory, G, Wickramasinghe, VO, Webb, AI, Phillippa TaberlayPhillippa Taberlay, Bromberg, KD, Lai, A, Papenfuss, AT, Smyth, GK, Allan, RS, Licht, JD, Landau, DA, Abdel-Wahab, O, Shortt, J, Vervoort, SJ, Johnstone, RWTo separate causal effects of histone acetylation on chromatin accessibility and transcriptional output, we used integrated epigenomic and transcriptomic analyses following acute inhibition of major cellular lysine acetyltransferases P300 and CBP in hematological malignancies. We found that catalytic P300/CBP inhibition dynamically perturbs steady-state acetylation kinetics and suppresses oncogenic transcriptional networks in the absence of changes to chromatin accessibility. CRISPR-Cas9 screening identified NCOR1 and HDAC3 transcriptional co-repressors as the principal antagonists of P300/CBP by counteracting acetylation turnover kinetics. Finally, deacetylation of H3K27 provides nucleation sites for reciprocal methylation switching, a feature that can be exploited therapeutically by concomitant KDM6A and P300/CBP inhibition. Overall, this study indicates that the steady-state histone acetylation-methylation equilibrium functions as a molecular rheostat governing cellular transcription that is amenable to therapeutic exploitation as an anti-cancer regimen.
History
Publication title
Molecular CellVolume
81Issue
10Pagination
2183-2200.e13ISSN
1097-2765Department/School
Tasmanian School of MedicinePublisher
Cell PressPlace of publication
United StatesRights statement
Crown Copyright 2021 Published by Elsevier Inc.Repository Status
- Restricted