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Flow cytometric analysis of cell division by dye dilution

Citation

Lyons, AB and Doherty, KV, Flow cytometric analysis of cell division by dye dilution, Current Protocols in Cytometry, John Wiley and Sons, New York, J. Paul Robinson (ed), New York, pp. 9.11.1-9.11.9. ISBN 0-471-16131-4 (1998) [Research Book Chapter]

Abstract

The technique described in this unit uses the intracellular fluorescent label carboxyfluorescein diacetate succinimidyl ester (CFSE) to tag proliferating cells. Covalently bound CFSE is divided equally between daughter cells, allowing discrimination of successive rounds of cell division. The technique is applicable to in vitro cell division as well as to in vivo division of adoptively transferred cells and can resolve up to eight successive generations. CFSE is long lived, permitting analysis for several months after transfer, and has the same spectral characteristics as fluorescein, so monoclonal antibodies conjugated to phycoerythrin or other compatible fluorochromes may be used to immunophenotype the dividing cells.

Item Details

Item Type:Research Book Chapter
Research Division:Medical and Health Sciences
Research Group:Immunology
Research Field:Immunology not elsewhere classified
Objective Division:Health
Objective Group:Clinical Health (Organs, Diseases and Abnormal Conditions)
Objective Field:Immune System and Allergy
Author:Lyons, AB (Dr Bruce Lyons)
Author:Doherty, KV (Dr Kathleen Doherty)
ID Code:14643
Year Published:1998
Deposited By:Pathology
Deposited On:1998-08-01
Last Modified:2017-02-10
Downloads:0

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