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Characterisation of a type IV pilus produced by Aeromonas caviae
journal contribution
posted on 2023-05-16, 11:21 authored by Kirov, SM, Sanderson, K, Tracey DicksonTracey DicksonA pilus produced by a clinical isolate of Aeromanas caviae (strain CA195) was purified and partially characterised. The M(r) of the pilin was estimated to be 23 kDa by SDS-PAGE. Its N-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type IV pili purified from other Aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. Bundle-forming pili, often with a polar location, were seen on the surface of strain CA195 which was highly adherent to HEp-2 cells. Removal of surface structures by mechanical means decreased adhesion (by ≤ 50%) suggesting that these pill played some role in HEp-2 cell binding. This pilus type could prove an important marker for enteropathogenic A. caviae which appear to lack other putative virulence factors.
History
Publication title
Journal of Medical MicrobiologyVolume
47Issue
6Pagination
527-531ISSN
0022-2615Department/School
Tasmanian School of MedicinePublisher
Pathological Society of Great Britain and IrelandPlace of publication
UKRepository Status
- Restricted