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Characterisation of a type IV pilus produced by Aeromonas caviae

journal contribution
posted on 2023-05-16, 11:21 authored by Kirov, SM, Sanderson, K, Tracey DicksonTracey Dickson
A pilus produced by a clinical isolate of Aeromanas caviae (strain CA195) was purified and partially characterised. The M(r) of the pilin was estimated to be 23 kDa by SDS-PAGE. Its N-terminal amino-acid sequence showed that it was closely related to 'bundle-forming' type IV pili purified from other Aeromonas spp. associated with gastro-enteritis and considered to be important intestinal colonisation factors. Bundle-forming pili, often with a polar location, were seen on the surface of strain CA195 which was highly adherent to HEp-2 cells. Removal of surface structures by mechanical means decreased adhesion (by ≤ 50%) suggesting that these pill played some role in HEp-2 cell binding. This pilus type could prove an important marker for enteropathogenic A. caviae which appear to lack other putative virulence factors.

History

Publication title

Journal of Medical Microbiology

Volume

47

Issue

6

Pagination

527-531

ISSN

0022-2615

Department/School

Tasmanian School of Medicine

Publisher

Pathological Society of Great Britain and Ireland

Place of publication

UK

Repository Status

  • Restricted

Socio-economic Objectives

Clinical health not elsewhere classified

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