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Methods for in vitro CRISPR/CasRx-mediated RNA editing


Chuang, Y-F and Wang, P-Y and Sarabajeet, SK and Lama, S and Lin, F-L and Liu, G, Methods for in vitro CRISPR/CasRx-mediated RNA editing, Frontiers in Cell and Developmental Biology pp. 1-13. ISSN 2296-634X (2021) [Refereed Article]

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Copyright Statement

Copyright 2021 Chuang, Wang, Kumar, Lama, Lin and Liu. This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) License. The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms

DOI: doi:10.3389/fcell.2021.667879


Specific changes in the genome have been accomplished by the revolutionary gene-editing tool known as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system. The advent of programmable RNA editing CRISPR/Cas nucleases has made this gene-editing tool safer and more precise. Specifically, CasRx, a family member of the Cas13d family, has shown great therapeutic potential. Here, we describe the in vitro methods of utilizing this powerful RNA editing platform and determine the RNA editing efficiencies for CasRx with different forms of guide RNAs (also known as gRNA or sgRNA).

Item Details

Item Type:Refereed Article
Keywords:CRISPR/Cas13d, CasRx, RNA editing, VEGF (Vascular endothelial growth factor), AAV (Adeno-associated virus)
Research Division:Biomedical and Clinical Sciences
Research Group:Medical biotechnology
Research Field:Gene and molecular therapy
Objective Division:Health
Objective Group:Clinical health
Objective Field:Treatment of human diseases and conditions
UTAS Author:Sarabajeet, SK (Mr Suraj Sarabajeet)
UTAS Author:Lama, S (Mr Suraj Lama)
UTAS Author:Lin, F-L (Dr Fan-Li Lin)
UTAS Author:Liu, G (Associate Professor Guei-Sheung Liu)
ID Code:146257
Year Published:2021
Web of Science® Times Cited:5
Deposited By:Menzies Institute for Medical Research
Deposited On:2021-08-26
Last Modified:2022-08-26
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