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The CYCLIN-DEPENDENT KINASE module of the mediator complex promotes flowering and reproductive development in pea
journal contribution
posted on 2023-05-21, 01:10 authored by Hasan, AM, Jacqueline Vander SchoorJacqueline Vander Schoor, Valerie HechtValerie Hecht, James WellerJames WellerControl of flowering time has been a major focus of comparative genetic analyses in plant development. This study reports on a forward genetic approach to define previously uncharacterized components of flowering control pathways in the long-day legume, pea (Pisum sativum). We isolated two complementation groups of late-flowering mutants in pea that define two uncharacterized loci, LATE BLOOMER3 (LATE3) and LATE4, and describe their diverse effects on vegetative and reproductive development. A map-based comparative approach was employed to identify the underlying genes for both loci, revealing that that LATE3 and LATE4 are orthologs of CYCLIN DEPENDENT KINASE8 (CDK8) and CYCLIN C1 (CYCC1), components of the CDK8 kinase module of the Mediator complex, which is a deeply conserved regulator of transcription in eukaryotes. We confirm the genetic and physical interaction of LATE3 and LATE4 and show that they contribute to the transcriptional regulation of key flowering genes, including the induction of the florigen gene FTa1 and repression of the floral repressor LF. Our results establish the conserved importance of the CDK8 module in plants and provide evidence for the function of CYCLIN C1 orthologs in the promotion of flowering and the maintenance of normal reproductive development.
Funding
Australian Research Council
History
Publication title
Plant PhysiologyVolume
182Pagination
1375-1386ISSN
0032-0889Department/School
School of Natural SciencesPublisher
Amer Soc Plant BiologistsPlace of publication
15501 Monona Drive, Rockville, USA, Md, 20855Rights statement
Copyright © 2020 American Society of Plant BiologistRepository Status
- Restricted