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Asperuloside enhances taste perception and prevents weight gain in high-fat fed mice


Ishaq, M and Tran, DT and Wu, Y and Nowak, K and Deans, BJ and Xin, JTZ and Loh, HL and Ng, WY and Yee, CW and Southam, B and Vicenzi, S and Randall, C and Yang, C and Tan, E and Pasupuleti, M and Grewal, AK and Ahmad, T and Shastri, M and Vicario, C and Ronci, M and Zuccarini, M and Bleasel, M and Scowen, P and Raffaeli, W and Da Andrea, G and Chellappan, DK and Jacobson, G and Bissember, AC and Smith, JA and Eri, R and Canales, J and Iglesias, M and Guven, N and Caruso, V, Asperuloside enhances taste perception and prevents weight gain in high-fat fed mice, Frontiers in Endocrinology, 12 pp. 1-11. ISSN 1664-2392 (2021) [Refereed Article]


Copyright Statement

Copyright © 2021 Ishaq, Tran, Wu, Nowak, Deans, Xin, Loh, Ng, Yee, Southam, Vicenzi, Randall, Yang, Tan, Pasupuleti, Grewal, Ahmad, Shastri, Vicario, Ronci, Zuccarini, Bleasel, Scowen, Raffaeli, D’Andrea, Chellappan, Jacobson, Bissember, Smith, Eri, Canales, Iglesias, Guven and Caruso. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License (

DOI: doi:10.3389/fendo.2021.615446


Asperuloside is an iridoid glycoside found in many medicinal plants that has produced promising anti-obesity results in animal models. In previous studies, three months of asperuloside administration reduced food intake, body weight, and adipose masses in rats consuming a high fat diet (HFD). However, the mechanisms by which asperuloside exerts its anti-obesity properties were not clarified. Here, we investigated homeostatic and nutrient-sensing mechanisms regulating food intake in mice consuming HFD. We confirmed the anti-obesity properties of asperuloside and, importantly, we identified some mechanisms that could be responsible for its therapeutic effect. Asperuloside reduced body weight and food intake in mice consuming HFD by 10.5 and 12.8% respectively, with no effect on mice eating a standard chow diet. Fasting glucose and plasma insulin were also significantly reduced. Mechanistically, asperuloside significantly reduced hypothalamic mRNA ghrelin, leptin, and pro-opiomelanocortin in mice consuming HFD. The expression of fat lingual receptors (CD36, FFAR1-4), CB1R and sweet lingual receptors (TAS1R2-3) was increased almost 2-fold by the administration of asperuloside. Our findings suggest that asperuloside might exert its therapeutic effects by altering nutrient-sensing receptors in the oral cavity as well as hypothalamic receptors involved in food intake when mice are exposed to obesogenic diets. This signaling pathway is known to influence the subtle hypothalamic equilibrium between energy homeostasis and reward-induced overeating responses. The present pre-clinical study demonstrated that targeting the gustatory system through asperuloside administration could represent a promising and effective new anti-obesity strategy.

Item Details

Item Type:Refereed Article
Keywords:CD36, FFAR1-4, TAS1R2-3, asperuloside, cannabinoid (CB) receptor 1, food intake, nutrient-sensing mechanisms, weight loss
Research Division:Biomedical and Clinical Sciences
Research Group:Pharmacology and pharmaceutical sciences
Research Field:Pharmaceutical sciences
Objective Division:Health
Objective Group:Clinical health
Objective Field:Treatment of human diseases and conditions
UTAS Author:Ishaq, M (Dr Muhammad Ishaq)
UTAS Author:Tran, DT (Miss Duyen Tran)
UTAS Author:Wu, Y (Miss Yue Wu)
UTAS Author:Nowak, K (Mr Krzysztof Nowak)
UTAS Author:Deans, BJ (Miss Bianca Deans)
UTAS Author:Southam, B (Mr Benjamin Southam)
UTAS Author:Vicenzi, S (Miss Silvia Vicenzi)
UTAS Author:Randall, C (Mr Cameron Randall)
UTAS Author:Yang, C ( Cecilia Yang)
UTAS Author:Tan, E (Dr Emma Tan)
UTAS Author:Pasupuleti, M (Ms Manideepika Pasupuleti)
UTAS Author:Grewal, AK (Mrs Avneet Grewal)
UTAS Author:Ahmad, T (Mr Tauseef Ahmad)
UTAS Author:Shastri, M (Mr Madhur Shastri)
UTAS Author:Bleasel, M (Dr Martin Bleasel)
UTAS Author:Scowen, P (Mr Paul Scowen)
UTAS Author:Jacobson, G (Professor Glenn Jacobson)
UTAS Author:Bissember, AC (Associate Professor Alex Bissember)
UTAS Author:Smith, JA (Associate Professor Jason Smith)
UTAS Author:Eri, R (Associate Professor Raj Eri)
UTAS Author:Canales, J (Professor Juan Canales)
UTAS Author:Iglesias, M (Dr Miguel Iglesias)
UTAS Author:Guven, N (Dr Nuri Guven)
UTAS Author:Caruso, V (Dr Vanni Caruso)
ID Code:145388
Year Published:2021
Web of Science® Times Cited:2
Deposited By:Health Sciences
Deposited On:2021-07-20
Last Modified:2022-03-24
Downloads:16 View Download Statistics

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